IJdo J W, Zhang Y, Hodzic E, Magnarelli L A, Wilson M L, Telford S R, Barthold S W, Fikrig E
Department of Internal Medicine, Yale University School of Medicine, and Connecticut Agricultural Experiment Station, New Haven 06520-4080, USA.
J Infect Dis. 1997 Sep;176(3):687-92. doi: 10.1086/514091.
The early antibody response in patients with human granulocytic ehrlichiosis (HGE) and in mice infected with the HGE agent was characterized by using sera to probe lysates of HL-60 cells infected with HGE organisms. Sera were obtained from 18 patients with HGE, mostly within the first 6 weeks of clinical infection, and from mice infected with the HGE agent for up to 3 weeks. A 44-kDa antigen was reactive with IgG in all 18 patients, and IgG to 40-, 65-, and 80-kDa antigens was present in 6, 8, and 7 patients, respectively. In addition, IgM to 40-, 44-, 65-, and 80-kDa antigens was found in 9, 5, 4, and 4 subjects. Immunoglobulins to antigens ranging between 95 and 125 kDa were detected less frequently. HGE agent-infected C3H/HeJ mice had an antibody response similar to that in humans. Thus, the 40- and 44-kDa proteins of the HGE agent elicit early strong antibody responses during infection. Characterization of the antigens recognized by antibodies during HGE should aid in diagnosis and understanding of the disease.
通过使用血清探测感染人粒细胞埃立克体病（HGE）病原体的HL-60细胞裂解物，对HGE患者和感染HGE病原体的小鼠的早期抗体反应进行了表征。血清取自18例HGE患者，大多在临床感染的前6周内，以及感染HGE病原体长达3周的小鼠。一种44 kDa的抗原在所有18例患者中均与IgG发生反应，分别有6例、8例和7例患者存在针对40 kDa、65 kDa和80 kDa抗原的IgG。此外，在9例、5例、4例和4例受试者中发现了针对40 kDa、44 kDa、65 kDa和80 kDa抗原的IgM。针对95至125 kDa之间抗原的免疫球蛋白检测频率较低。感染HGE病原体的C3H/HeJ小鼠具有与人类相似的抗体反应。因此，HGE病原体的40 kDa和44 kDa蛋白在感染期间引发早期强烈的抗体反应。表征HGE期间抗体识别的抗原应有助于疾病的诊断和理解。
