登革4型病毒的分子进化与系统发育

Molecular evolution and phylogeny of dengue-4 viruses.

作者信息

Lanciotti R S, Gubler D J, Trent D W

机构信息

Division of Vector-Borne Infectious Diseases, Centers for Disease Control and Prevention, Public Health Service, US Department of Health and Human Services, Fort Collins, CO 80522, USA. rs12@CIDVBI 1.EM.CDC.GOV

出版信息

J Gen Virol. 1997 Sep;78 ( Pt 9):2279-84. doi: 10.1099/0022-1317-78-9-2279.

DOI:10.1099/0022-1317-78-9-2279

PMID:9292015

Abstract

Nucleotide sequences of the envelope protein genes of 19 geographically and temporally distinct dengue (DEN)-4 viruses were determined. Nucleic acid sequence comparison revealed that the identity among the DEN-4 viruses was greater than 92%. Similarity among deduced amino acids was between 96 and 100%; in most cases identical amino acid substitutions occurred among viruses from similar geographical regions. Alignment of nucleic acid sequences followed by parsimony analysis generated phylogenetic trees, which indicated that geographically independent evolution of DEN-4 viruses had occurred. DEN-4 viruses were separated into two genetically distinct subtypes (genotypes). Genotype-1 contains viruses from the Philippines, Thailand and Sri Lanka; genotype-2 consists of viruses from Indonesia, Tahiti, the Caribbean Islands (Puerto Rico, Dominica) and Central and South America.

摘要

测定了19株在地理和时间上不同的登革4型（DEN-4）病毒包膜蛋白基因的核苷酸序列。核酸序列比较显示，DEN-4病毒之间的同一性大于92%。推导氨基酸之间的相似性在96%至100%之间；在大多数情况下，来自相似地理区域的病毒之间发生相同的氨基酸替换。对核酸序列进行比对并随后进行简约分析生成了系统发育树，这表明DEN-4病毒发生了地理上独立的进化。DEN-4病毒被分为两个遗传上不同的亚型（基因型）。基因型1包含来自菲律宾、泰国和斯里兰卡的病毒；基因型2由来自印度尼西亚、塔希提岛、加勒比群岛（波多黎各、多米尼克）以及中美洲和南美洲的病毒组成。