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小鼠生精小管固有层中周细胞样肌样细胞和成纤维细胞中NADPH-黄递酶活性的超微结构显示

Ultrastructural demonstration of the NADPH-diaphorase activity in peritubular myoid cells and fibroblasts of the lamina propria of the mouse seminiferous tubules.

作者信息

Ruffoli R, Menchini Fabris F, Giambelluca M A, Giannessi F

机构信息

Istituto di Anatomia Umana Normale, Facoltà di Medicina e Chirurgia dell'Università degli Studi di Pisa, Italy.

出版信息

In Vivo. 1997 Jul-Aug;11(4):319-24.

PMID:9292298
Abstract

The NOS-related NADPH-diaphorase activity was studied by transmission electron microscopy in the peritubular myoid cells and fibroblasts of normal mouse testis. The reaction product was observed on the membranes of the endoplasmic reticulum, on the Golgi apparatus and nuclear envelope. The peritubular myoid cells and fibroblasts showed similar ultracytochemical features; the intensity of the enzymatic reaction was suggestive of an important role of the NOS/cGMP enzymatic system in these cells. Some hypotheses on the role of NO in the peritubular myoid cells and fibroblasts are proposed.

摘要

通过透射电子显微镜研究了正常小鼠睾丸曲细精管周围肌样细胞和成纤维细胞中与一氧化氮合酶(NOS)相关的NADPH-黄递酶活性。在内质网、高尔基体和核膜上观察到反应产物。曲细精管周围肌样细胞和成纤维细胞显示出相似的超细胞化学特征;酶反应强度提示NOS/cGMP酶系统在这些细胞中起重要作用。提出了一些关于一氧化氮在曲细精管周围肌样细胞和成纤维细胞中作用的假说。

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