Vaz A J, Nakamura P M, Camargo M E, Camargo E D, Ferreira A W
Faculdude de Ciências-Farmacêculicas, Universidade de São Paulo, Brasil.
Rev Inst Med Trop Sao Paulo. 1996 Nov-Dec;38(6):391-6. doi: 10.1590/s0036-46651996000600001.
A dot-ELISA was developed for the detection of antibodies in CSF in the immunologic diagnosis of human neurocysticercosis, using antigen extracts of the membrane and scolex of Cysticercus cellulosae (M+S-Cc) and, alternately, membrane (M) and vesicular fluid (VF) of Cysticercus longicollis (Cl) covalently bound to a new solid phase consisting of polyester fabric treated with N-methylol-acrylamide resin (dot-RT). The test was performed at room temperature, with reduced incubation times and with no need for special care in the manipulation of the support. The sensitivity rates obtained were 95.1% for antigen Cc and 97.6% for antigen Cl. Specificity was 90.6% when Cc was used, and 96.9% and 100% when M-Cl and VF-Cl were used, respectively. No significant differences in titer were observed between tests carried out with homologous and heterologous antigens. The low cost and easy execution of the dot-RT test using antigen extracts of Cysticercus longicollis indicate the test for use in the immunodiagnosis of human neurocysticercosis.
开发了一种斑点酶联免疫吸附测定法（dot-ELISA），用于在人类神经囊尾蚴病的免疫诊断中检测脑脊液中的抗体，该方法使用猪囊尾蚴（M+S-Cc）的膜和头节的抗原提取物，以及交替使用长膜壳绦虫（Cl）的膜（M）和囊泡液（VF），它们共价结合到由用N-羟甲基丙烯酰胺树脂处理的聚酯织物组成的新固相上（dot-RT）。该测试在室温下进行，孵育时间缩短，并且在操作载体时无需特别小心。使用抗原Cc时获得的灵敏度为95.1%，使用抗原Cl时为97.6%。使用Cc时特异性为90.6%，使用M-Cl和VF-Cl时分别为96.9%和100%。使用同源和异源抗原进行的测试之间在滴度上未观察到显著差异。使用长膜壳绦虫抗原提取物的dot-RT测试成本低且易于执行，表明该测试可用于人类神经囊尾蚴病的免疫诊断。
