头颈部表皮样癌中P53的过表达

P53 overexpression in epidermoid carcinoma of the head and neck.

作者信息

Logullo A F, Kowalski L P, Nonogaki S, Miguel R E, Torloni H, Brentani R R

机构信息

Ludwig Institute for Cancer Research, Department of Head and Neck Surgery, A.C. Camargo Hospital, Fundação Antonio Prudente, São Paulo, Brazil.

出版信息

Sao Paulo Med J. 1997 Jan-Feb;115(1):1349-55. doi: 10.1590/s1516-31801997000100006.

DOI:10.1590/s1516-31801997000100006

Abstract

UNLABELLED

The theory of field cancerization in tumors of the head and neck reflects the complex oncogenesis that occurs in this region. The mechanisms that control cell proliferation at the molecular level in epidermoid carcinomas (ECs) of the upper aerodigestive tract are still unclear. Mutations in p53 are the genetic alterations most often detected in ECs of the head and neck and seem to contribute actively to the carcinogenic process triggered by p53 as a tumor-suppressor gene and to its association with tobacco. The objective of the present study was to investigate the expression of p53 protein in epidermoid head and neck carcinomas by immunohistochemistry and its immunohistochemical correlation with other prognostic factors. The study was conducted on 63 consecutive ECs cases not submitted to previous treatment. Specimens of the tumor and of the normal adjacent mucosa were collected during surgery and submitted to immunohistochemical reaction for the determination of the expression of anti-protein p53 antibody (M7001 DAKO A/S, Denmark). Anatomo-clinical and demographic data were not significantly correlated with the presence of lymph node metastases or p53 expression in the tumor or in the adjacent normal mucosa. Tumor localization in the larynx was significantly correlated with p53 expression. Histological grading as grades I, II, III and IV was correlated with significant p53 expression (p = 0.025).

CONCLUSIONS

in the studied material obtained from 63 cases of head and neck ECs, we detected a 48 percent rate of immunohistochemically detectable p53 overexpression; 2) we did not detect a relationship between demographic patient data and p53 expression in the tumor or in the normal adjacent mucosa; 3) p53 overexpression was significantly more frequent in ECs material from the larynx: and 4) The presence of 12 cases with p53 overexpression in the normal adjacent mucosa and with a p53-negative tumor is in agreement with the theory of field cancerization. Follow-up of this patient series for a longer period of time will permit a better analysis of these values.

摘要

未标记

头颈部肿瘤的场癌化理论反映了该区域发生的复杂肿瘤发生过程。上呼吸道消化道表皮样癌（EC）中在分子水平控制细胞增殖的机制仍不清楚。p53突变是头颈部EC中最常检测到的基因改变，似乎作为肿瘤抑制基因的p53所触发的致癌过程及其与烟草的关联中发挥积极作用。本研究的目的是通过免疫组织化学研究p53蛋白在头颈部表皮样癌中的表达及其与其他预后因素的免疫组织化学相关性。该研究对63例未经先前治疗的连续EC病例进行。手术期间收集肿瘤和相邻正常黏膜的标本，并进行免疫组织化学反应以测定抗p53蛋白抗体（丹麦DAKO A/S公司的M7001）的表达。解剖临床和人口统计学数据与肿瘤或相邻正常黏膜中淋巴结转移的存在或p53表达无显著相关性。肿瘤位于喉部与p53表达显著相关。组织学分级为I、II、III和IV级与显著的p53表达相关（p = 0.025）。

结论

1）在从63例头颈部EC获得的研究材料中，我们检测到免疫组织化学可检测到的p53过表达率为48%；2）我们未检测到患者人口统计学数据与肿瘤或相邻正常黏膜中p53表达之间的关系；3）p53过表达在喉部EC材料中明显更频繁；4）12例在相邻正常黏膜中有p53过表达且肿瘤为p53阴性的病例符合场癌化理论。对该患者系列进行更长时间的随访将有助于更好地分析这些值。