Martin J L, Cross G F
Department of Microbiology, Monash Medical School, Alfred Hospital, Prahran, Victoria.
Aust Vet J. 1997 Aug;75(8):579-82. doi: 10.1111/j.1751-0813.1997.tb14198.x.
The objective of this study is to compare the strain of chlamydia causing genital infection in koalas from Victoria with isolates from other animal species.
Polymerase chain reaction and restriction enzyme analysis has been used to compare various Chlamydia psittaci isolates from a range of animals and disease syndromes. The isolates used in this study include isolates from three birds, three from aborted sheep, one from polyarthritis, one from bovine abortion, one from feline pneumonitis, three porcine isolates from faeces, polyarthritis and abortion, and three urogenital isolates from Victorian koalas.
Two polymerase chain reactions were performed, each amplifying a different region of the omp 1 gene. The first polymerase chain reaction amplified a 144 bp segment of the gene which was then digested with the restriction enzyme EcoR I. The second polymerase chain reaction amplified a larger 1070 bp region of the omp 1 gene which was digested with two restriction enzymes Alu I and Nde II.
The results obtained have confirmed that variation in DNA sequence of various animal chlamydia isolates does occur. They have also shown that it is possible to classify isolates, based on their restriction enzyme profiles, into distinct groups.
本研究的目的是比较来自维多利亚州考拉身上引起生殖器感染的衣原体菌株与其他动物物种的分离株。
聚合酶链反应和限制性酶切分析已被用于比较来自一系列动物和疾病综合征的各种鹦鹉热衣原体分离株。本研究中使用的分离株包括来自三只鸟类的分离株、来自流产绵羊的三株、来自多关节炎的一株、来自牛流产的一株、来自猫肺炎的一株、来自粪便、多关节炎和流产的三株猪分离株，以及来自维多利亚州考拉的三株泌尿生殖道分离株。
进行了两次聚合酶链反应，每次扩增omp 1基因的不同区域。第一次聚合酶链反应扩增了该基因的一个144 bp片段，然后用限制性酶EcoR I进行消化。第二次聚合酶链反应扩增了omp 1基因一个更大的1070 bp区域，并用两种限制性酶Alu I和Nde II进行消化。
获得的结果证实了各种动物衣原体分离株的DNA序列确实存在变异。结果还表明，根据其限制性酶切图谱将分离株分类为不同的组是可能的。
