de Wolde H, Pruim J, Mastik M F, Koudstaal J, Molenaar W M
Department of Pathology and PET Center, University and University Hospital of Groningen, The Netherlands.
J Nucl Med. 1997 Sep;38(9):1369-74.
To validate the protein synthesis rate (PSR) measured in human brain tumors using L-[1-(11)C]tyrosine (TYR) PET, the PSR was compared to histopathological parameters that reflect proliferation and protein synthesis.
We studied 20 patients who had a brain biopsy and who also underwent a PET study with TYR. Paraffin sections were stained with the monoclonal antibody MIB 1, targeted against the core antigen Ki-67, and nucleolar organizer regions (NORs) were measured as argyrophilic NORs (AgNORs). The TYR uptake was measured by PET, and with a kinetic model, the PSR was determined.
PSR (nmol/ml/min) ranged from 0.44 to 1.99 (mean, 0.97), Ki-67 labeling indices (%) ranged from 0.9 to 33.5 (mean, 9.5) and AgNOR area (mm2/cm2) ranged from 0.13 to 0.85. No relationship was found between PSR and Ki-67 labeling index or AgNOR area.
It seems that the PSR and proliferation, as measured by Ki-67, are independent processes. The role of the PSR is uncertain, but it is likely that it can be seen as a marker for the homeostasis of the cell.
为验证使用L-[1-(11)C]酪氨酸（TYR）PET测量人脑肿瘤中的蛋白质合成率（PSR），将PSR与反映增殖和蛋白质合成的组织病理学参数进行比较。
我们研究了20例接受脑活检且同时接受TYR PET研究的患者。石蜡切片用针对核心抗原Ki-67的单克隆抗体MIB 1染色，并测量核仁组织区（NORs）作为嗜银NORs（AgNORs）。通过PET测量TYR摄取，并使用动力学模型确定PSR。
PSR（nmol/ml/分钟）范围为0.44至1.99（平均值，0.97），Ki-67标记指数（%）范围为0.9至33.5（平均值，9.5），AgNOR面积（mm2/cm2）范围为0.13至0.85。未发现PSR与Ki-67标记指数或AgNOR面积之间存在相关性。
似乎PSR与通过Ki-67测量的增殖是独立的过程。PSR的作用尚不确定，但很可能它可被视为细胞内稳态的标志物。
