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过氧化物胁迫下大肠杆菌谷氧还蛋白基因表达的氧抗性调节蛋白（OxyR）依赖性诱导

oxyR-dependent induction of Escherichia coli grx gene expression by peroxide stress.

作者信息

Tao K

机构信息

Radioisotope Center, University of Tokyo, Japan.

出版信息

J Bacteriol. 1997 Sep;179(18):5967-70. doi: 10.1128/jb.179.18.5967-5970.1997.

DOI:10.1128/jb.179.18.5967-5970.1997
PMID:9294462
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC179494/
Abstract

The Escherichia coli OxyR protein is a transcriptional activator for a number of genes induced in response to low concentrations of hydrogen peroxide. To identify additional OxyR-regulated genes, I cloned a DNA fragment that shows promoter activity regulated by OxyR by direct selection of OxyR-binding DNA fragments. Analyses of the cloned fragment indicate that the grx gene, encoding glutaredoxin 1, is inducible by hydrogen peroxide in an oxyR-dependent fashion.

摘要

大肠杆菌的OxyR蛋白是一种转录激活因子，可激活许多因低浓度过氧化氢诱导而表达的基因。为了鉴定其他受OxyR调控的基因，我通过直接筛选与OxyR结合的DNA片段，克隆了一个显示受OxyR调控的启动子活性的DNA片段。对该克隆片段的分析表明，编码谷氧还蛋白1的grx基因可被过氧化氢以依赖OxyR的方式诱导表达。

相似文献

1
oxyR-dependent induction of Escherichia coli grx gene expression by peroxide stress.过氧化物胁迫下大肠杆菌谷氧还蛋白基因表达的氧抗性调节蛋白（OxyR）依赖性诱导
J Bacteriol. 1997 Sep;179(18):5967-70. doi: 10.1128/jb.179.18.5967-5970.1997.
2
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J Biochem. 1991 Feb;109(2):262-6.
3
Activation of the OxyR transcription factor by reversible disulfide bond formation.通过可逆二硫键形成激活OxyR转录因子。
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本文引用的文献

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Identification and characterization of hydrogen peroxide-sensitive mutants of Escherichia coli: genes that require OxyR for expression.大肠杆菌过氧化氢敏感突变体的鉴定与表征：表达需要OxyR的基因。
J Bacteriol. 1997 Jan;179(2):330-8. doi: 10.1128/jb.179.2.330-338.1997.
2
Redox-dependent shift of OxyR-DNA contacts along an extended DNA-binding site: a mechanism for differential promoter selection.OxyR与DNA的结合沿扩展的DNA结合位点发生氧化还原依赖性移位：一种差异启动子选择机制。
Cell. 1994 Sep 9;78(5):897-909. doi: 10.1016/s0092-8674(94)90702-1.
3
The dps promoter is activated by OxyR during growth and by IHF and sigma S in stationary phase.dps启动子在生长过程中由OxyR激活，在稳定期由整合宿主因子（IHF）和σS激活。
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