神经肽Y通过与参与神经肽Y相位转移不同的受体来对抗垂体腺苷酸环化酶激活肽的相位转移。

NPY opposes PACAP phase shifts via receptors different from those involved in NPY phase shifts.

作者信息

Harrington M E, Hoque S

机构信息

Department of Psychology, Smith College, Northampton, MA 01063, USA.

出版信息

Neuroreport. 1997 Aug 18;8(12):2677-80. doi: 10.1097/00001756-199708180-00008.

DOI:10.1097/00001756-199708180-00008

PMID:9295099

Abstract

The suprachiasmatic nuclei (SCN) of the hamster can be maintained for several days in vitro, allowing electrophysiological investigation of the mammalian circadian clock. Application of pituitary adenylate cyclase activating peptide (PACAP) at Zeitgeber time (ZT) 6 on the first day in vitro can phase shift the rhythm of firing rate expressed by SCN neurons on a subsequent day in vitro. Here we report that co-application of neuropeptide Y (NPY) will block the phase-shifting action of PACAP. This blocking action is mimicked by [Leu31,Pro34]NPY and [D-Trp32]NPY but not by NPY(22-36) or avian pancreatic polypeptide. The results indicate that NPY has actions in the SCN via receptors distinct from the Y2 receptor, which mediates the phase-shifting action of NPY.

摘要

仓鼠的视交叉上核（SCN）可在体外维持数天，这使得对哺乳动物生物钟进行电生理研究成为可能。在体外培养的第一天，于授时因子时间（ZT）6施加垂体腺苷酸环化酶激活肽（PACAP），可使SCN神经元在后续体外培养日所表现出的放电频率节律发生相位偏移。在此我们报告，共同施加神经肽Y（NPY）会阻断PACAP的相位偏移作用。[Leu31,Pro34]NPY和[D-Trp32]NPY可模拟这种阻断作用，但NPY(22 - 36)或禽胰多肽则不能。结果表明，NPY通过与介导NPY相位偏移作用的Y2受体不同的受体在SCN中发挥作用。