Elger B, Laux V, Schwarz M
Knoll AG, Ludwigshafen, Germany.
Arzneimittelforschung. 1997 Aug;47(8):895-9.
In vivo magnetic resonance imaging was used to study the effect of ancrod (CAS 9046-56-4, Arwin), a plasma fibrinogen level lowering agent, on brain lesion in two rat models of acute focal cerebral ischaemia. Total lesion volume was determined by multislice T2-weighted magnetic resonance imaging 24 h after permanent middle cerebral artery occlusion. Intravenous infusion of ancrod starting 30 min after middle cerebral artery occlusion at dosages of 10, 30, 50 or 70 IU/kg (n = 9/group) significantly diminished cerebral lesion volume by 20 to 33% as compared to vehicle-infused controls (n = 12). None of the ancrod-treated rats showed evidence of intracerebral bleeding on T2-weighted magnetic resonance images taken after 24 h. In photochemically induced (rose bengal) unilateral thrombotic cortical infarction brain damage was displayed by multislice diffusion-weighted magnetic resonance imaging after 24 h. Again, post treatment with ancrod reduced total volume of cerebral lesion dose-dependently from 142 +/- 28 mm3 in the controls (n = 10) to 121 +/- 28 mm3 (n = 10) and 111 +/- 20 mm3 (n = 11, p < 0.05) in rats treated with 10 and 30 IU/kg ancrod, respectively (means +/- S.D.). These results suggest cerebroprotection in focal cerebral ischaemia by improvements in the cerebral microcirculation which may offer a potential and safe approach for therapy of acute stroke.
采用体内磁共振成像技术，研究血浆纤维蛋白原水平降低剂安克洛酶（CAS 9046 - 56 - 4，Arwin）对两种急性局灶性脑缺血大鼠模型脑损伤的影响。在永久性大脑中动脉闭塞24小时后，通过多层T2加权磁共振成像确定总损伤体积。大脑中动脉闭塞30分钟后开始静脉输注安克洛酶，剂量为10、30、50或70 IU/kg（每组n = 9），与输注赋形剂的对照组（n = 12）相比，脑损伤体积显著减少20%至33%。在24小时后拍摄的T2加权磁共振图像上，接受安克洛酶治疗的大鼠均未显示脑内出血迹象。在光化学诱导（孟加拉玫瑰红）的单侧血栓性皮质梗死中，24小时后通过多层扩散加权磁共振成像显示脑损伤情况。同样，安克洛酶治疗后，脑损伤总体积呈剂量依赖性降低，对照组（n = 10）为142±28 mm³，接受10 IU/kg和30 IU/kg安克洛酶治疗的大鼠分别为121±28 mm³（n = 10）和111±20 mm³（n = 11，p < 0.05）（均值±标准差）。这些结果表明，通过改善脑微循环对局灶性脑缺血具有脑保护作用，这可能为急性中风的治疗提供一种潜在且安全的方法。
