Baeyens W, Goutier R
Strahlentherapie. 1977 Nov;153(11):769-73.
Rats were sacrificed at different times after injection of 14C-orotate and liver nuclei were isolated and incubated in a cell-free system made of soluble cytoplasmic fraction from non-irradiated rat livers. The release of ribonucleoproteins from labelled nuclei to the incubation medium was measured on nuclei from normal and total-body irradiated rats (500 to 2000 R). When using short labelling times in vivo (30 min), which would label predominantly messenger-type RNP, the nuclear transport activity was depressed at early times (3 h) after whole-body irradiation, but increased at later times (24 h). When the labelling time in vivo is increased to 2 h, no significant difference was observed between control and irradiated groups. Transport of the stable ribosomal-type RNP, observed 24 h after both labelling and irradiation, was depressed. These results are discussed in view of previous personal studies on radiation effect on liver RNA synthesis and polyribosomes formation in vivo.
在注射14C-乳清酸后的不同时间处死大鼠，分离肝细胞核，并在由未受照射的大鼠肝脏的可溶性细胞质部分制成的无细胞系统中孵育。测量正常大鼠和全身照射大鼠（500至2000拉德）的细胞核中核糖核蛋白从标记细胞核释放到孵育培养基中的情况。当在体内使用短标记时间（30分钟）时，这主要标记信使型核糖核蛋白，全身照射后早期（3小时）核转运活性降低，但后期（24小时）增加。当体内标记时间增加到2小时时，对照组和照射组之间未观察到显著差异。标记和照射24小时后观察到的稳定核糖体型核糖核蛋白的转运受到抑制。结合之前关于辐射对体内肝脏RNA合成和多核糖体形成影响的个人研究对这些结果进行了讨论。
