Honma Y, Iwanaga M
Department of Bacteriology, Faculty of Medicine, University of the Ryukyus, Okinawa, Japan.
FEMS Microbiol Lett. 1997 Sep 1;154(1):111-6. doi: 10.1111/j.1574-6968.1997.tb12631.x.
BT23, a Vibrio cholerae O1 E1 Tor isolate, possesses the cholera toxin (CT) gene as determined by PCR. However, CT was not detected in the culture medium by the reversed passive latex agglutination test, nor in the whole cell lysate as examined by Western blotting. The toxin-coregulated pilus (TCP) was not detected by Western blotting. This suggests the presence of defects in the regulatory cascade. toxR, toxS and toxT, members of the regulatory cascade, were examined by PCR. toxR and toxS were conserved but toxT was not. CT and TCP production was complemented by transformation of toxT. The lack of toxT was suspected to be the cause of the undetectable production of CT in strain BT23.
BT23是一株霍乱弧菌O1 El Tor分离株，经聚合酶链反应（PCR）检测确定其拥有霍乱毒素（CT）基因。然而，通过反向被动乳胶凝集试验在培养基中未检测到CT，通过蛋白质免疫印迹法（Western blotting）在全细胞裂解物中也未检测到CT。通过蛋白质免疫印迹法未检测到毒素协同调节菌毛（TCP）。这表明调节级联存在缺陷。通过PCR检测了调节级联的成员toxR、toxS和toxT。toxR和toxS是保守的，但toxT不是。通过toxT转化补充了CT和TCP的产生。怀疑toxT的缺失是BT23菌株中CT产生未被检测到的原因。
