Fey S J, Nawrocki A, Larsen M R, Görg A, Roepstorff P, Skews G N, Williams R, Larsen P M
Centre for Proteome Analysis in Life Sciences, International Science Park Odense, Denmark.
Electrophoresis. 1997 Aug;18(8):1361-72. doi: 10.1002/elps.1150180811.
Proteome analysis offers a unique means of identifying important proteins, characterizing their modifications and beginning to describe their function. This is achieved through the combination of two technologies: protein separation and selection by two-dimensional gel electrophoresis, and protein identification and characterization by mass spectrometry. This methodological outline sketches the strengths and weaknesses of the two central technologies used, and provides both practical tips and the theoretical background for their utilization. One application of these technologies is illustrated by the characterization of genes, revealed by sequencing, but which have no--or only weak homology--to any other known genes. Other applications, for example the identification of protein markers for particular human diseases, are only referred to. The aim of the article is thus to provide the basis for a sound understanding of the full potential and limitations of proteome analysis.
蛋白质组分析提供了一种独特的方法来鉴定重要蛋白质、表征其修饰并初步描述其功能。这是通过两种技术的结合来实现的：二维凝胶电泳进行蛋白质分离和筛选，以及质谱法进行蛋白质鉴定和表征。本方法概述勾勒了所使用的两种核心技术的优缺点，并为其应用提供了实用技巧和理论背景。这些技术的一个应用通过对测序揭示的基因进行表征来说明，这些基因与任何其他已知基因没有或只有微弱的同源性。其他应用，例如鉴定特定人类疾病的蛋白质标志物，仅作提及。因此，本文的目的是为全面理解蛋白质组分析的全部潜力和局限性提供基础。
