Li J, Chory J
Plant Biology Laboratory, The Salk Institute for Biological Studies, La Jolla, California 92037, USA.
Cell. 1997 Sep 5;90(5):929-38. doi: 10.1016/s0092-8674(00)80357-8.
Brassinosteroids are a class of growth-promoting regulators that play a key role throughout plant development. Despite their importance, nothing is known of the mechanism of action of these steroid hormones. We describe the identification of 18 Arabidopsis dwarf mutants that are unable to respond to exogenously added brassinosteroid, a phenotype that might be expected for brassinosteroid signaling mutants. All 18 mutations define alleles of a single previously described gene, BRI1. We cloned BRI1 and examined its expression pattern. It encodes a ubiquitously expressed putative receptor kinase. The extracellular domain contains 25 tandem leucine-rich repeats that resemble repeats found in animal hormone receptors, plant disease resistance genes, and genes involved in unknown signaling pathways controlling plant development.
油菜素甾醇是一类促进生长的调节剂，在植物整个发育过程中发挥关键作用。尽管它们很重要，但对于这些甾体激素的作用机制却一无所知。我们描述了18个拟南芥矮化突变体的鉴定，这些突变体对外源添加的油菜素甾醇无反应，这种表型可能是油菜素甾醇信号突变体所预期的。所有18个突变都定义了一个先前描述的单一基因BRI1的等位基因。我们克隆了BRI1并检查了它的表达模式。它编码一种普遍表达的假定受体激酶。细胞外结构域包含25个串联的富含亮氨酸的重复序列，类似于在动物激素受体、植物抗病基因以及参与控制植物发育的未知信号通路的基因中发现的重复序列。
