Bartlett D, Glaser M, Welti R
Division of Biology, Kansas State University, Manhattan 66506, USA.
Biochim Biophys Acta. 1997 Aug 14;1328(1):48-54. doi: 10.1016/s0005-2736(97)00072-2.
The dansyl fluors of 1-oleoyl-2-[4-(dansyl)amino]butyl]-sn-glycero-3-phosphocholine (18:1,4-dansyl PC) and 1-palmitoyl-2-[1-[(dansyl)amino)]undecanoyl]-sn-glycero-3-phosphoc holine (16:0,11-dansyl PC) were shown to reside at similar depths in phosphatidylcholine vesicles at pH 7. Analysis of fluorescence emission maxima showed that the dansyl groups of both 18:1,4-dansyl PC and 16:0,11-dansyl PC in phosphatidylcholine vesicles experienced environments more polar than methanol, suggesting that the dansyl group attached to the terminus of the undecanoyl chain must fold back toward the bilayer surface. Fluorescence polarization measurements in solid/fluid lipid mixtures show that both probes partition strongly into fluid phase lipid. The very low polarization values of 16:0,11-dansyl PC in lipid vesicles are consistent with the notion that the dansyl fluor of 16:0,11-dansyl PC existed in an environment allowing a high degree of motional freedom due to folding back of the dansyl group attached to the undecanoyl chain.
1-油酰基-2-[4-(丹磺酰基)氨基]丁基]-sn-甘油-3-磷酸胆碱（18:1,4-丹磺酰基PC）和1-棕榈酰基-2-[1-[(丹磺酰基)氨基)]十一烷酰基]-sn-甘油-3-磷酸胆碱（16:0,11-丹磺酰基PC）的丹磺酰荧光团在pH 7的磷脂酰胆碱囊泡中处于相似深度。对荧光发射最大值的分析表明，磷脂酰胆碱囊泡中18:1,4-丹磺酰基PC和16:0,11-丹磺酰基PC的丹磺酰基团所处环境比甲醇更具极性，这表明连接在十一烷酰链末端的丹磺酰基团必定折向双层表面。在固体/流体脂质混合物中的荧光偏振测量表明，两种探针都强烈分配到流体相脂质中。脂质囊泡中16:0,11-丹磺酰基PC的极低偏振值与以下观点一致：由于连接在十一烷酰链上的丹磺酰基团折回，16:0,11-丹磺酰基PC的丹磺酰荧光团存在于一个允许高度运动自由度的环境中。
