Owen T J, Lantz G C, Hiles M C, VanVleet J, Martin B R, Geddes L A
Department of Veterinary Clinical Sciences, School of Veterinary Medicine, West Lafayette, Indiana 47907, USA.
J Surg Res. 1997 Aug;71(2):179-86. doi: 10.1006/jsre.1997.5148.
Glutaraldehyde treatment of collagen biomaterials promotes calcification, poor host-tissue incorporation, and ultimately mechanical failure of bioprotheses. Porcine small-intestinal submucosa (SIS) is a biomaterial which has been investigated for several applications including arterial and venous grafts and repair of tendon, ligament, body wall, and urinary bladder defects. The calcification potential of peracetic acid (PAA)-sterilized SIS was studied. Four test samples, (1) native (cleaned, untreated) SIS, (2) SIS sterilized with 0.1% PAA, (3) SIS treated with 0.25% glutaraldehyde for 20 min, and (4) commercially available glutaraldehyde-preserved porcine bioprosthetic heart valve cusp segments (GPV), were each implanted subcutaneously in each of 24 weanling rats. Six rats were euthanatized at 1, 2, 4, and 8 weeks. Evaluation of calcium concentration by atomic absorption spectroscopy and extent of mineralization and fibrosis by light microscopy were performed. Atomic absorption revealed no calcification in native or peracetic acid-treated SIS at any time point compared with preimplant calcium concentration. Statistically significant (P < 0.0001) calcification occurred in glutaraldehyde-treated materials (SIS and GPV) at each evaluation as compared to native and peracetic acid-treated samples. Histopathology indicated native and peracetic acid-treated SIS showed no implant mineralization (P < 0.0001) and little peri-implant fibrosis (P < 0.0001). Results suggested that native and peracetic acid-treated SIS have a low calcification potential and further study of this biomaterial is warranted.
戊二醛处理胶原蛋白生物材料会促进钙化、宿主组织整合不良，并最终导致生物假体的机械故障。猪小肠黏膜下层（SIS）是一种生物材料，已被研究用于多种应用，包括动静脉移植以及肌腱、韧带、体壁和膀胱缺损的修复。研究了过氧乙酸（PAA）灭菌的SIS的钙化潜力。将四个测试样品，（1）天然（清洁、未处理）SIS，（2）用0.1% PAA灭菌的SIS，（3）用0.25%戊二醛处理20分钟的SIS，以及（4）市售的戊二醛保存的猪生物人工心脏瓣膜尖段（GPV），分别皮下植入24只断奶大鼠体内。在1、2、4和8周时对六只大鼠实施安乐死。通过原子吸收光谱法评估钙浓度，并通过光学显微镜评估矿化和纤维化程度。原子吸收显示，与植入前的钙浓度相比，天然或过氧乙酸处理的SIS在任何时间点均未出现钙化。与天然和过氧乙酸处理的样品相比，在每次评估时，戊二醛处理的材料（SIS和GPV）均出现了具有统计学意义（P < 0.0001）的钙化。组织病理学表明，天然和过氧乙酸处理的SIS未显示植入物矿化（P < 0.0001），且植入物周围几乎没有纤维化（P < 0.0001）。结果表明，天然和过氧乙酸处理的SIS具有较低的钙化潜力，值得对这种生物材料进行进一步研究。
