Ohmi K, Masuda T, Yamaguchi H, Sakurai T, Kudo Y, Katsuki M, Nonomura Y
Department of 1st Pharmacology, Faculty of Medicine, University of Tokyo, Japan.
Biochem Biophys Res Commun. 1997 Sep 8;238(1):154-8. doi: 10.1006/bbrc.1997.7218.
Here we report that we could obtain a highly differentiated smooth muscle cell line by screening the expression of a-smooth muscle actin from p53 knook out mice aorta. This cell revealed extended bipolar shape and expressed h-caldesmon and calponin as well as a-smooth muscle actin as protein markers of differentiated smooth muscle. Further intracellular calcium increase was induced by application of noradrenaline in a dose dependent manner and calcium oscillation was also observed in a higher dose (100 microM). Appropriate application of 5-azacytidine enhanced these tendencies and induced slow contraction by endothelin-1 and phenylephrine.
在此我们报告，通过筛选p53基因敲除小鼠主动脉中α-平滑肌肌动蛋白的表达，我们能够获得一种高度分化的平滑肌细胞系。这种细胞呈现出延长的双极形态，表达h-钙调蛋白和钙调素以及α-平滑肌肌动蛋白，作为分化平滑肌的蛋白质标志物。去甲肾上腺素以剂量依赖性方式诱导细胞内钙进一步增加，并且在较高剂量（100微摩尔）时也观察到钙振荡。适当应用5-氮杂胞苷增强了这些趋势，并诱导内皮素-1和去氧肾上腺素引起缓慢收缩。
