Laffargue M, Monnereau L, Tuech J, Ragab A, Ragab-Thomas J, Payrastre B, Raynal P, Chap H
INSERM U326, IFR30, Hôpital Purpan, Toulouse, France.
Biochem Biophys Res Commun. 1997 Sep 8;238(1):247-51. doi: 10.1006/bbrc.1997.7269.
Using a specific polyclonal anti-Tec antibody, we have shown that Tec is expressed in human platelets. In addition, Tec was found to undergo tyrosine phosphorylation during platelet activation. The phosphorylation increased after 1 min and remained stable after 3 min of thrombin treatment. The tetrapeptide RGDS inhibited more than 90% of thrombin-induced tyrosine phosphorylation of Tec and blocked its translocation to the cytoskeleton. These results suggest that Tec participates in platelet signaling downstream of integrin activation.
