Rufini S, Rainaldi G, Abbracchio M P, Fiorentini C, Capri M, Franceschi C, Malorni W
Department of Biology, University of Rome Tor Vergata, via della Ricerca Scientifica, Rome, 00133, Italy.
Biochem Biophys Res Commun. 1997 Sep 18;238(2):361-6. doi: 10.1006/bbrc.1997.7287.
Recent results for various cell types have shown that adenosine is capable of inducing relevant cytophatological alterations which can eventually lead to apoptosis. No data are available regarding the involvement of adenosine in apoptosis of muscle cells. In this work, we studied the effect of the relatively hydrolysis-resistant adenosine analog 2-chloro adenosine on a cultured myogenic cell line, C2C12, which is able to differentiate in vitro, leading to the formation of syncythia, giant multinucleated cells called myotubes. Results indicated that 2-chloro adenosine induces apoptotic cell death in both myoblasts and myotubes; this was preceded by a derangement of actin microfilaments, which represent the main cytoskeletal component and play a pivotal role in the physiology of such cell line. The time-dependency of cytoskeletal alterations suggested a causal relationship between these changes and the induction of apoptosis. These results implicate adenosine as an endogenous regulator of apoptosis in muscle cells and validate this cell model system as a useful tool for studying human muscle cell pathologies.
最近针对各种细胞类型的研究结果表明，腺苷能够诱导相关的细胞病理学改变，最终可能导致细胞凋亡。目前尚无关于腺苷参与肌肉细胞凋亡的相关数据。在本研究中，我们研究了相对抗水解的腺苷类似物2-氯腺苷对一种可体外分化、能形成多核巨细胞即肌管的成肌细胞系C2C12的影响。结果表明，2-氯腺苷可诱导成肌细胞和肌管发生凋亡性细胞死亡；在此之前，肌动蛋白微丝会发生紊乱，肌动蛋白微丝是主要的细胞骨架成分，在此细胞系的生理学过程中起关键作用。细胞骨架改变的时间依赖性表明这些变化与细胞凋亡诱导之间存在因果关系。这些结果表明腺苷是肌肉细胞凋亡的内源性调节因子，并验证了该细胞模型系统是研究人类肌肉细胞病理学的有用工具。
