Lalani E N, Stubbs A, Stamp G W
Department of Histopathology, Royal Postgraduate Medical School, London, UK.
Semin Cancer Biol. 1997 Feb;8(1):53-9. doi: 10.1006/scbi.1997.0052.
Current research into prostate cancer increasingly demands greater input from pathologists. There is a requirement for improved morphological assessment, classification and grading of neoplasia. The provision of optimally preserved material and establishment of tissue 'banks' is vital to facilitate molecular biological analysis. Microdissection of archival material can provide a source of relatively pure DNA and mRNA which can 'be further amplified by PCR/RT-PCR. This enables allelic imbalance, point mutations and other genetic abnormalities to be demonstrated. In-situ hybridization for mRNA is feasible on fixed tissues and allows precise localization of gene expression on complex tissues or for labile gene products. Experimental models including transgenic mice and in-vitro co-culture systems require sophisticated morphological analysis. Experts in morphological analysis are essential members of basic scientific and translational research teams.
目前对前列腺癌的研究越来越需要病理学家提供更多的投入。对肿瘤的形态学评估、分类和分级需要改进。提供保存最佳的材料并建立组织“库”对于促进分子生物学分析至关重要。对存档材料进行显微切割可提供相对纯净的DNA和mRNA来源，这些DNA和mRNA可通过PCR/RT-PCR进一步扩增。这能够证明等位基因失衡、点突变和其他基因异常。mRNA原位杂交在固定组织上是可行的，并且能够在复杂组织或不稳定基因产物上精确地定位基因表达。包括转基因小鼠和体外共培养系统在内的实验模型需要精密的形态学分析。形态学分析专家是基础科学和转化研究团队的重要成员。
