Gerberick G F, Cruse L W, Miller C M, Sikorski E E, Ridder G M
Miami Valley Laboratories, The Procter and Gamble Company, Cincinnati, Ohio 45253-8707, USA.
Toxicol Appl Pharmacol. 1997 Sep;146(1):1-10. doi: 10.1006/taap.1997.8218.
Naive and activated T cells are known to express different adhesion molecules and are thought to exhibit different migratory patterns that result from their expression of discrete adhesion molecules. Two adhesion molecules that have been associated with differentiating naive and activated/memory T cells are CD62L (L-selectin) and CD44 (H-CAM). It has been demonstrated previously that naive T cells express a CD62LhiCD44lo phenotype, whereas memory T cells exhibit a CD62LloCD44hi phenotype. The purpose of the present investigation was to determine whether chemical allergens, in contrast to irritants, would induce a CD62LloCD44hi phenotype on CD4 and/or CD8 T cells isolated from draining lymph nodes (DLN) of treated mice. Mice were treated on the ears for 3 consecutive days with concentrations of allergens or irritants which caused an increase in the number of DLN cells. The DLN were excised 72 hr following the final chemical treatment and cells prepared for analysis by flow cytometry. In mice treated with the allergen trinitrochlorobenzene an increase in the percentage of CD4+ cells expressing CD62LloCD44(hi) was observed compared to cells isolated from mice treated with the irritant benzalkonium chloride or vehicle treated mice. Mice treated with dintrochlorobenzene had an increase in the percentage of CD4+ cells expressing CD62LloCD44(hi) that was dose dependent and peaked at 72 hr following the final allergen treatment. Concomitant with changes on CD4+ cells, increases in the percentage of CD8+ cells expressing CD62LloCD44hi were observed with allergens, but not with irritants. Increases in the percentage of CD4+ and CD8+ cells expressing CD62LloCD44(hi) were observed with other allergens including oxazolone and alpha-hexylcinnamaldehyde, but not the irritant sodium lauryl sulfate. These data demonstrate that allergens, but not irritants, cause a selective and reproducible increase in the percentage of CD4+ and CD8+ cells expressing the T cell activation/memory phenotype CD62LloCD44hi. Analysis of T cell activation/memory markers may be useful in differentiating allergen and irritant responses in the draining lymph nodes of chemically treated mice.
已知初始T细胞和活化T细胞表达不同的黏附分子，并且被认为由于它们表达不同的黏附分子而表现出不同的迁移模式。与区分初始T细胞和活化/记忆T细胞相关的两种黏附分子是CD62L（L-选择素）和CD44（H-CAM）。先前已经证明，初始T细胞表达CD62L高CD44低表型，而记忆T细胞表现出CD62L低CD44高表型。本研究的目的是确定与刺激物相比，化学变应原是否会在从经处理小鼠的引流淋巴结（DLN）分离的CD4和/或CD8 T细胞上诱导CD62L低CD44高表型。用导致DLN细胞数量增加的变应原或刺激物浓度连续3天处理小鼠耳部。在最后一次化学处理后72小时切除DLN，并制备细胞用于流式细胞术分析。与用刺激物苯扎氯铵处理的小鼠或用赋形剂处理的小鼠分离的细胞相比，在用变应原三硝基氯苯处理的小鼠中，观察到表达CD62L低CD44（高）的CD4 +细胞百分比增加。用二硝基氯苯处理的小鼠中，表达CD62L低CD44（高）的CD4 +细胞百分比增加，呈剂量依赖性，并在最后一次变应原处理后72小时达到峰值。与CD4 +细胞的变化同时，在用变应原处理的小鼠中观察到表达CD62L低CD44高的CD8 +细胞百分比增加，但在用刺激物处理的小鼠中未观察到。在用其他变应原（包括恶唑酮和α-己基肉桂醛）处理的小鼠中观察到表达CD62L低CD44（高）的CD4 +和CD8 +细胞百分比增加，但在用刺激物十二烷基硫酸钠处理的小鼠中未观察到。这些数据表明，变应原而非刺激物会导致表达T细胞活化/记忆表型CD62L低CD44高的CD4 +和CD8 +细胞百分比选择性且可重复地增加。分析T细胞活化/记忆标志物可能有助于区分化学处理小鼠引流淋巴结中的变应原和刺激物反应。
