生长因子对人结肠黏膜上皮细胞体外修复的影响。

Effect of growth factors on epithelial restitution of human colonic mucosa in vitro.

作者信息

Riegler M, Sedivy R, Sogukoglu T, Cosentini E, Bischof G, Teleky B, Feil W, Schiessel R, Hamilton G, Wenzl E

机构信息

University Clinic of Surgery, University of Vienna, Austria.

出版信息

Scand J Gastroenterol. 1997 Sep;32(9):925-32. doi: 10.3109/00365529709011204.

DOI:10.3109/00365529709011204

PMID:9299673

Abstract

BACKGROUND

Epithelial restitution enables resurfacing of epithelial discontinuities by enterocyte migration. This study investigated the effect of basic fibroblast growth factor (bFGF), insulin-like growth factor (IGF-1), and epidermal growth factor (EGF) on restitution of human colonic mucosa in vitro.

METHODS

After base-line incubation human colonic mucosal strips, mounted in Ussing chambers, were luminally exposed to 0.5 mM sodium deoxycholate (NaDOC) for 10 min. Thereafter tissues were incubated with buffer alone or luminal buffer containing various concentrations of bFGF, IGF-1, and EGF for 3 h. Resistance (R) was calculated from potential difference (PD) and short-circuit current (Isc). All tissues were processed for light microscopy. Extent of damage was measured by morphometry.

RESULTS

Luminal 0.5 mM NaDOC for 10 min caused R to drop by 43% (n = 4; P < 0.05). Compared with controls 50 ng/ml EGF induced an approximately 30% R increase until the end of the experiments (P < 0.05, n = 4, paired). Ten minutes after injury 50.2 +/- 4% of the mucosa was damaged (n = 6), and after 3 h damage was significantly reduced by EGF (17.2 +/- 3% versus 31.7 +/- 4%, 50 ng/ml EGF versus controls) (P < 0.05, n = 6 per group). Histology showed that EGF stimulated enterocyte migration over the basal lamina. Various doses of bFGF and IGF-1 did not impair restitution when compared with controls.

CONCLUSION

In contrast to bFGF and IGF-1, EGF was shown to promote epithelial restitution of human colonic mucosa in vitro.

摘要

背景

上皮修复通过肠上皮细胞迁移实现上皮连续性的重新形成。本研究调查了碱性成纤维细胞生长因子（bFGF）、胰岛素样生长因子（IGF-1）和表皮生长因子（EGF）对人结肠黏膜体外修复的影响。

方法

在基线孵育后，将安装在尤斯灌流小室中的人结肠黏膜条向腔内暴露于0.5 mM脱氧胆酸钠（NaDOC）10分钟。此后，组织分别在单独的缓冲液或含有不同浓度bFGF、IGF-1和EGF的腔内缓冲液中孵育3小时。通过电位差（PD）和短路电流（Isc）计算电阻（R）。所有组织均进行光镜检查。通过形态计量学测量损伤程度。

结果

腔内0.5 mM NaDOC处理10分钟导致R下降43%（n = 4；P < 0.05）。与对照组相比，50 ng/ml EGF使R增加约30%，直至实验结束（P < 0.05，n = 4，配对）。损伤后10分钟，50.2±4%的黏膜受损（n = 6），3小时后，EGF使损伤显著减轻（50 ng/ml EGF组为17.2±3%，对照组为31.7±4%）（P < 0.05，每组n = 6）。组织学显示，EGF刺激肠上皮细胞在基膜上迁移。与对照组相比，不同剂量的bFGF和IGF-1均未损害修复。