Houston C T, Reilly J P
Department of Chemistry, Indiana University, Bloomington 47405, USA.
Rapid Commun Mass Spectrom. 1997;11(13):1435-9. doi: 10.1002/(SICI)1097-0231(19970830)11:13<1435::AID-RCM24>3.0.CO;2-A.
Matrix-assisted laser desorption time-of-flight mass spectrometry (MALDI-TOFMS) is applied to the analysis of whole human blood. No separation or purification steps are required to observe individual globins and the sensitivity of the method is excellent. Mass accuracy and precision are 0.01% using a linear TOF system with pulsed ion extraction. Sickle-cell hemoglobin is easily distinguishable from normal hemoglobin. The speed and simplicity of the method make it potentially attractive for large-scale population screening.
基质辅助激光解吸飞行时间质谱法（MALDI-TOFMS）被应用于全血分析。无需进行分离或纯化步骤即可观察到各个珠蛋白，且该方法的灵敏度极佳。使用具有脉冲离子提取功能的线性TOF系统时，质量准确度和精密度为0.01%。镰状细胞血红蛋白很容易与正常血红蛋白区分开来。该方法的速度和简便性使其在大规模人群筛查中具有潜在吸引力。
