果蝇抗菌肽（drosomycin）的溶液结构，昆虫中的首个可诱导抗真菌蛋白。
Solution structure of drosomycin, the first inducible antifungal protein from insects.
作者信息
Landon C, Sodano P, Hetru C, Hoffmann J, Ptak M
机构信息
Centre de Biophysique Moléculaire (UPR 4301 CNRS), Orléans, France.
出版信息
Protein Sci. 1997 Sep;6(9):1878-84. doi: 10.1002/pro.5560060908.
Abstract
Drosomycin is the first antifungal protein characterized recently among the broad family of inducible peptides and proteins produced by insects to respond to bacterial or septic injuries. It is a small protein of 44 amino acid residues extracted from Drosophila melanogaster that exhibits a potent activity against filamentous fungi. Its three-dimensional structure in aqueous solution was determined using 1H 2D NMR. This structure, involving an alpha-helix and a twisted three-stranded beta-sheet, is stabilized by three disulfide bridges. The corresponding Cysteine Stabilized alpha beta (CS alpha beta) motif, which was found in other defense proteins such as the antibacterial insect defensin A, short- and long-chain scorpion toxins, as well as in plant thionins and potent antifungal plant defensins, appears as remarkably persistent along evolution.
摘要
果蝇霉素是昆虫产生的用于应对细菌或脓毒症损伤的可诱导肽和蛋白质这一广泛家族中最近鉴定出的首个抗真菌蛋白。它是一种从黑腹果蝇中提取的由44个氨基酸残基组成的小蛋白，对丝状真菌具有强大活性。利用1H 2D NMR确定了其在水溶液中的三维结构。该结构包含一个α螺旋和一个扭曲的三链β折叠片层，由三个二硫键稳定。相应的半胱氨酸稳定化αβ（CSαβ）基序，在其他防御蛋白如抗菌昆虫防御素A、短链和长链蝎毒素以及植物硫素和强效抗真菌植物防御素中也有发现，在进化过程中表现出显著的持久性。
相似文献
1
Solution structure of drosomycin, the first inducible antifungal protein from insects.果蝇抗菌肽（drosomycin）的溶液结构，昆虫中的首个可诱导抗真菌蛋白。
Protein Sci. 1997 Sep;6(9):1878-84. doi: 10.1002/pro.5560060908.
2
The active site of drosomycin, a small insect antifungal protein, delineated by comparison with the modeled structure of Rs-AFP2, a plant antifungal protein.通过与植物抗真菌蛋白Rs-AFP2的模拟结构进行比较，确定了小型昆虫抗真菌蛋白果蝇霉素的活性位点。
J Pept Res. 2000 Oct;56(4):231-8. doi: 10.1034/j.1399-3011.2000.00757.x.
3
Determination of the disulfide array of the first inducible antifungal peptide from insects: drosomycin from Drosophila melanogaster.昆虫中首个可诱导抗真菌肽的二硫键排列的确定：黑腹果蝇的果蝇抗菌肽
FEBS Lett. 1996 Oct 14;395(1):6-10. doi: 10.1016/0014-5793(96)00992-1.
4
Solution structure of thanatin, a potent bactericidal and fungicidal insect peptide, determined from proton two-dimensional nuclear magnetic resonance data.通过质子二维核磁共振数据确定的强效杀菌和杀真菌昆虫肽thanatin的溶液结构。
Eur J Biochem. 1998 Sep 1;256(2):404-10. doi: 10.1046/j.1432-1327.1998.2560404.x.
5
Solution structure of termicin, an antimicrobial peptide from the termite Pseudacanthotermes spiniger.来自白蚁（Pseudacanthotermes spiniger）的抗菌肽——白蚁素的溶液结构
Protein Sci. 2003 Mar;12(3):438-46. doi: 10.1110/ps.0228303.
6
Drosomycin, an essential component of antifungal defence in Drosophila.果蝇霉素，果蝇抗真菌防御的重要组成部分。
Insect Mol Biol. 2009 Oct;18(5):549-56. doi: 10.1111/j.1365-2583.2009.00907.x.
7
1H-NMR-derived secondary structure and the overall fold of the potent anti-mammal and anti-insect toxin III from the scorpion Leiurus quinquestriatus quinquestriatus.源自1H-NMR的蝎子以色列金蝎强效抗哺乳动物和抗昆虫毒素III的二级结构及整体折叠
Eur J Biochem. 1996 Mar 1;236(2):395-404. doi: 10.1111/j.1432-1033.1996.00395.x.
8
NMR structure and conformational dynamics of AtPDFL2.1, a defensin-like peptide from Arabidopsis thaliana.拟南芥防御素样肽AtPDFL2.1的核磁共振结构与构象动力学
Biochim Biophys Acta. 2016 Dec;1864(12):1739-1747. doi: 10.1016/j.bbapap.2016.08.017. Epub 2016 Aug 31.
9
The three-dimensional solution structure of NaD1, a new floral defensin from Nicotiana alata and its application to a homology model of the crop defense protein alfAFP.来自烟草的新型花卉防御素NaD1的三维溶液结构及其在作物防御蛋白alfAFP同源模型中的应用。
J Mol Biol. 2003 Jan 3;325(1):175-88. doi: 10.1016/s0022-2836(02)01103-8.
10
Functional divergence of six isoforms of antifungal peptide Drosomycin in Drosophila melanogaster.黑腹果蝇中抗真菌肽果蝇霉素六种同工型的功能差异
Gene. 2006 Sep 1;379:26-32. doi: 10.1016/j.gene.2006.03.017. Epub 2006 May 20.
引用本文的文献
1
Biochemistry, Mechanistic Intricacies, and Therapeutic Potential of Antimicrobial Peptides: An Alternative to Traditional Antibiotics.抗菌肽的生物化学、机制复杂性和治疗潜力：传统抗生素的替代物。
Curr Med Chem. 2024;31(37):6110-6139. doi: 10.2174/0109298673268458230926105224.
2
In Silico Analysis of a Parasitoid Venom Peptide Reveals Prevalence of the Cation-Polar-Cation Clip Motif in Knottin Proteins.对一种寄生蜂毒液肽的计算机模拟分析揭示了结蛋白中阳离子-极性-阳离子剪辑基序的普遍性。
Pathogens. 2023 Jan 14;12(1):143. doi: 10.3390/pathogens12010143.
3
Mutation-driven evolution of antibacterial function in an ancestral antifungal scaffold: Significance for peptide engineering.抗菌功能在祖先抗真菌支架中的突变驱动进化：对肽工程的意义。
Front Microbiol. 2022 Dec 1;13:1053078. doi: 10.3389/fmicb.2022.1053078. eCollection 2022.
4
Transgenic Silkworms Overexpressing Relish and Expressing Drosomycin Confer Enhanced Immunity to Multiple Pathogens.过表达Relish并表达果蝇抗菌肽的转基因家蚕对多种病原体具有增强的免疫力。
Mol Biotechnol. 2022 Jun;64(6):711-724. doi: 10.1007/s12033-021-00438-0. Epub 2022 Jan 31.
5
Analysis of a gene family for PDF-like peptides from Arabidopsis.拟南芥 PDF 样肽基因家族的分析。
Sci Rep. 2021 Sep 23;11(1):18948. doi: 10.1038/s41598-021-98175-6.
6
Antimicrobial Peptides: A New Hope in Biomedical and Pharmaceutical Fields.抗菌肽：生物医药与制药领域的新希望。
Front Cell Infect Microbiol. 2021 Jun 14;11:668632. doi: 10.3389/fcimb.2021.668632. eCollection 2021.
7
Membrane-Interacting Antifungal Peptides.膜相互作用抗真菌肽
Front Cell Dev Biol. 2021 Apr 12;9:649875. doi: 10.3389/fcell.2021.649875. eCollection 2021.
8
Plant Defensins from a Structural Perspective.从结构角度看植物防御素
Int J Mol Sci. 2020 Jul 26;21(15):5307. doi: 10.3390/ijms21155307.
9
Structure-Activity Relationships of Insect Defensins.昆虫防御素的构效关系
Front Chem. 2017 Jul 12;5:45. doi: 10.3389/fchem.2017.00045. eCollection 2017.
10
Antifungal Activity against Filamentous Fungi of Ts1, a Multifunctional Toxin from Scorpion Venom.蝎子毒液中的多功能毒素Ts1对丝状真菌的抗真菌活性
Front Microbiol. 2017 Jun 6;8:984. doi: 10.3389/fmicb.2017.00984. eCollection 2017.
本文引用的文献
1
Determination of the disulfide array of the first inducible antifungal peptide from insects: drosomycin from Drosophila melanogaster.昆虫中首个可诱导抗真菌肽的二硫键排列的确定：黑腹果蝇的果蝇抗菌肽
FEBS Lett. 1996 Oct 14;395(1):6-10. doi: 10.1016/0014-5793(96)00992-1.
2
1H-NMR-derived secondary structure and the overall fold of the potent anti-mammal and anti-insect toxin III from the scorpion Leiurus quinquestriatus quinquestriatus.源自1H-NMR的蝎子以色列金蝎强效抗哺乳动物和抗昆虫毒素III的二级结构及整体折叠
Eur J Biochem. 1996 Mar 1;236(2):395-404. doi: 10.1111/j.1432-1033.1996.00395.x.
3
Structure-activity analysis of thanatin, a 21-residue inducible insect defense peptide with sequence homology to frog skin antimicrobial peptides.兔防御肽（兔抗菌肽）的结构-活性分析，一种由21个氨基酸组成的可诱导昆虫防御肽，与蛙皮抗菌肽具有序列同源性。
Proc Natl Acad Sci U S A. 1996 Feb 6;93(3):1221-5. doi: 10.1073/pnas.93.3.1221.
4
1H-nmr studies on the structure of a new thionin from barley endosperm.关于大麦胚乳中一种新硫堇结构的核磁共振氢谱研究
Biopolymers. 1995 Dec;36(6):751-63. doi: 10.1002/bip.360360608.
5
Determination of the three-dimensional solution structure of noxiustoxin: analysis of structural differences with related short-chain scorpion toxins.诺氏毒素三维溶液结构的测定：与相关短链蝎毒素的结构差异分析。
Biochemistry. 1995 Dec 26;34(51):16563-73. doi: 10.1021/bi00051a004.
6
A new family of basic cysteine-rich plant antifungal proteins from Brassicaceae species.来自十字花科植物的一类新的富含半胱氨酸的碱性植物抗真菌蛋白。
FEBS Lett. 1993 Feb 1;316(3):233-40. doi: 10.1016/0014-5793(93)81299-f.
7
Solution structure of gamma 1-H and gamma 1-P thionins from barley and wheat endosperm determined by 1H-NMR: a structural motif common to toxic arthropod proteins.通过核磁共振氢谱确定的来自大麦和小麦胚乳的γ1-H和γ1-P硫堇蛋白的溶液结构：一种有毒节肢动物蛋白共有的结构基序
Biochemistry. 1993 Jan 19;32(2):715-24. doi: 10.1021/bi00053a041.
8
A new approach to analysis and display of local lipophilicity/hydrophilicity mapped on molecular surfaces.一种分析和展示分子表面局部亲脂性/亲水性图谱的新方法。
J Comput Aided Mol Des. 1993 Oct;7(5):503-14. doi: 10.1007/BF00124359.
9
Three-dimensional structure of ectatomin from Ectatomma tuberculatum ant venom.来自结节切叶蚁毒液的ectatomin的三维结构。
J Biomol NMR. 1995 Jan;5(1):1-13. doi: 10.1007/BF00227465.
10
Insect immunity. Septic injury of Drosophila induces the synthesis of a potent antifungal peptide with sequence homology to plant antifungal peptides.
J Biol Chem. 1994 Dec 30;269(52):33159-63.
Zotero 插件