利用7-氟-4-硝基苯并-2-恶唑-1,3-二唑进行荧光标记开发一种灵敏的血浆中ABT-089定量方法。
Development of a sensitive method for quantitation of ABT-089 in plasma using fluorescence labeling with 7-fluoro-4-nitrobenzo-2-oxa-1,3-diazole.
作者信息
Hui Y H, Carroll S, Marsh K C
机构信息
Drug Analysis, Abbott Laboratories, Abbott Park, IL 60064, USA.
出版信息
J Chromatogr B Biomed Sci Appl. 1997 Aug 1;695(2):337-47. doi: 10.1016/s0378-4347(97)00162-x.
A high-performance liquid chromatography method for the quantitation of ABT-089 [2-methyl-3-(2-(S)-pyrrolidinylmethoxy)pyridine] (I), a new structural type of cholinergic channel modulators (ChCM), is described in this paper using 7-fluoro-4-nitrobenzo-2-oxa-1,3-diazole (NBD-F) as a fluorescent-labeling reagent. The method combined an optimized liquid-liquid extraction from plasma followed by pre-column derivatization to yield a fluorescence product. The selectivity, sensitivity, and reproducibility of this method were found to be excellent. This method was applied to the determination of ng/ml plasma and tissue levels of ABT-089 and similar compounds in biological samples.
本文描述了一种高效液相色谱法，用于定量分析新型结构类型的胆碱能通道调节剂（ChCM）ABT-089 [2-甲基-3-(2-(S)-吡咯烷基甲氧基)吡啶]（I），该方法使用7-氟-4-硝基苯并-2-恶唑-1,3-二唑（NBD-F）作为荧光标记试剂。该方法结合了从血浆中优化的液-液萃取，然后进行柱前衍生化以产生荧光产物。结果发现该方法的选择性、灵敏度和重现性都非常出色。该方法应用于测定生物样品中ABT-089及类似化合物的血浆和组织水平，检测限可达纳克/毫升。
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