Cooper J D, Shearsby N, Sheung C T
Clinical Innovations Ltd., Kenilworth, Warwickshire, UK.
J Chromatogr B Biomed Sci Appl. 1997 Aug 15;696(1):117-22. doi: 10.1016/s0378-4347(97)00104-7.
The use of the system automated sequential trace enrichment of dialysates (ASTED), to prepare plasma samples for the estimation of L-NG-monomethylarginine (546C88) by pre-column o-phthalaldehyde-2-mercaptoethanol derivatisation and HPLC is described. Calibration is achieved using purified albumin as a substitute matrix for plasma. Using this technique the procedure was observed to be specific for 546C88 and linear over the range 0.10 to 50.0 mumol/l. The within-run imprecision (C.V.) at four different spiked plasma 546C88 concentrations of 0.10, 1.0, 8.0 and 40.0 mumol/l was 6.48, 2.55, 2.79 and 3.37%, respectively, and the between-run imprecision (C.V.) estimated to be 8.50, 1.80, 2.10 and 3.30%, respectively, for the same spiked 546C88 concentrations. The overall accuracy (% bias) of the procedure using an albumin matrix for calibration was estimated to be -2.50, -5.25, -3.56, -3.53%, respectively, and the recovery of 546C88 from six different spiked plasma samples estimated to be 99.1 +/- 1.4%.
描述了使用透析液系统自动化顺序痕量富集（ASTED），通过柱前邻苯二甲醛 - 2 - 巯基乙醇衍生化和高效液相色谱法制备血浆样品以估计L - NG - 单甲基精氨酸（546C88）的方法。使用纯化白蛋白作为血浆的替代基质进行校准。使用该技术观察到该方法对546C88具有特异性，并且在0.10至50.0μmol / l范围内呈线性。在0.10、1.0、8.0和40.0μmol / l的四种不同加标血浆546C88浓度下，批内不精密度（变异系数）分别为6.48%、2.55%、2.79%和3.37%，对于相同的加标546C88浓度，批间不精密度（变异系数）估计分别为8.50%、1.80%、2.10%和3.30%。使用白蛋白基质进行校准的该方法的总体准确度（偏差百分比）估计分别为 - 2.50%、 - 5.25%、 - 3.56%、 - 3.53%，并且从六个不同加标血浆样品中546C88的回收率估计为99.1±1.4%。
