Immunoaffinity column cleanup procedure for analysis of ivermectin in swine liver. - Suppr | 超能文献

Immunoaffinity column cleanup procedure for analysis of ivermectin in swine liver.

作者信息

Li J S, Li X W, Hu H B

机构信息

Department of Veterinary Pharmacology, Pharmacy and Toxicology, College of Veterinary Medicine, China Agricultural University, Beijing, People's Republic of China.

出版信息

J Chromatogr B Biomed Sci Appl. 1997 Aug 15;696(1):166-71. doi: 10.1016/s0378-4347(97)00220-x.

DOI:10.1016/s0378-4347(97)00220-x

Abstract

A simple and sensitive method for the analysis of ivermectin (22,23-dihydroavermectin B1) in swine liver based on immunoaffinity column cleanup is described. The immunosorbent was prepared by coupling polyclonal anti-ivermectin antibodies to carbonyl diimidazole-activated Sepharose CL-4B. After extraction with methanol, ivermectin was cleaned up on an immunoaffinity column, and determined by reversed-phase liquid chromatography with UV absorbance detection at 245 nm. Recoveries of ivermectin from fortified samples of 5-10 micrograms kg-1 levels ranged 85-102%, with coefficients of variation of 6-12%. The limit of detection was 2 micrograms kg-1 in a 5-g samples.

摘要

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