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牛蛙交感神经元细胞膜的电生理研究。

Electrophysiological studies of the cell membrane of bullfrog sympathetic neurons.

作者信息

Tokimasa T

机构信息

Department of Physiology, School of Medicine, Tokai University, Isehara Kanagawa, Japan.

出版信息

Tokai J Exp Clin Med. 1996 Dec;21(4-6):157-63.

PMID:9300975
Abstract

Active and passive properties of dissociated bullfrog sympathetic ganglion cells were examined in the whole-cell configuration. Electrical constants were 107 k omega cm2 and 1 microFcm2 for the unitary cell membrane area. Under conditions in which the equilibrium potential was 55 mV for sodium ions and -101 mV for potassium ions, the action potential and its after-hyperpolarization peaked at 50 and -83 mV, respectively, when elicited from the resting potential of -63 mV. These observations are consistent with those reported previously for other autonomic neurons. The reversal potential of the after-hyperpolarization was approximately 10 mV less negative than the potassium equilibrium potential. The basis of this difference has been discussed using a hypothetical equivalent circuit.

摘要

采用全细胞记录模式研究了牛蛙离体交感神经节细胞的主动和被动特性。单位细胞膜面积的电学常数分别为107 kΩ·cm²和1 μF·cm²。在钠离子平衡电位为55 mV、钾离子平衡电位为 -101 mV的条件下,当从 -63 mV的静息电位诱发动作电位时,其峰值为50 mV,动作电位后的超极化峰值为 -83 mV。这些观察结果与先前报道的其他自主神经元的结果一致。动作电位后超极化的反转电位比钾离子平衡电位负约10 mV。已使用假设的等效电路讨论了这种差异的基础。

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