Watanabe G, Umetsu K, Sato M, Suzuki T
Department of Forensic Medicine, Yamagata University School of Medicine, Japan.
Nihon Hoigaku Zasshi. 1997 Jun;51(3):205-10.
We found the AG allele in three cases (two cases are AGOA type and one case is A1AG type) out of unrelated 150 Japanese by amplified product length polymorphism technique for genotyping of ABO blood group system. The AG alleles amplified with allele-specific PCR method, were analyzed from exon 6 to 7 by direct sequencing. The AG allele was divided into suballees, AG1 and AG2. The AG1 and AG2 were revealed to have the same sequence as B allele in exon 6. In exon 7, however, the AG1 allele had the same sequence as A1 allele and the AG2 allele had hybrid sequence of B and OG allele.
我们通过扩增产物长度多态性技术对ABO血型系统进行基因分型，在150名无关的日本人群中发现了3例AG等位基因（2例为AGOA型，1例为A1AG型）。采用等位基因特异性PCR方法扩增得到的AG等位基因，通过直接测序从外显子6到7进行分析。AG等位基因被分为AG1和AG2两个亚等位基因。AG1和AG2在外显子6中的序列与B等位基因相同。然而，在外显子7中，AG1等位基因的序列与A1等位基因相同，AG2等位基因具有B和OG等位基因的杂交序列。
