Bay B H
Department of Anatomy, National University of Singapore, Kent Ridge, Singapore.
Okajimas Folia Anat Jpn. 1997 May;74(1):33-7. doi: 10.2535/ofaj1936.74.1_33.
Extracellular ATP has been previously reported to induce cell retraction and stimulate DNA synthesis in human Chang liver cells via phosphoinositide (PI) signal transduction. The ATP-treated cell assumes a rounded morphology and is tethered to the substratum by microvillous anchors as demonstrated in this study by scanning electron and confocal microscopy. The addition of 0.5 mM genistein, which is known to completely abolish extracellular ATP-stimulated DNA synthesis in Chang liver cells, did not inhibit ATP-induced cell rounding. The results suggest that the seemingly conjoint responses of ATP-stimulated DNA synthesis and cell rounding could be dissociated.
先前已有报道称，细胞外ATP可通过磷酸肌醇（PI）信号转导诱导人张氏肝细胞收缩并刺激其DNA合成。如本研究通过扫描电子显微镜和共聚焦显微镜所示，经ATP处理的细胞呈现圆形形态，并通过微绒毛锚定与基质相连。添加0.5 mM金雀异黄素（已知其可完全消除张氏肝细胞中细胞外ATP刺激的DNA合成）并未抑制ATP诱导的细胞变圆。结果表明，ATP刺激的DNA合成和细胞变圆这两种看似联合的反应可能是可分离的。
