Tanaka S
Osaka Red Cross Blood Center.
Nihon Rinsho. 1997 Sep;55(9):2277-81.
Irradiation of blood products is an effective way to prevent post-transfusion graft versus host disease (PT-GVHD). We examined the mitotic activity of lymphocytes in blood products with the aid of 3H-thymidine uptake after irradiation with various doses of X-ray. The results showed the mixed lymphocyte culture (MLC) response was completely abolished by irradiation with only 5 Gy. Irradiation with 50 Gy completely eliminated mitogen-stimulated 3H-thymidine uptake (Mitogen response). The risk of PT-GVHD would be eliminated by irradiation with over 15 Gy, because MLC response seems to reflect closely the mechanism of PT-GVHD in patients. Furthermore, it was shown that X-ray irradiation with up to 50 Gy has no deleterious effects on in vitro characteristics of blood products. However, we must pay attention to the high concentration of potassium in the plasma of blood products stored after irradiation.
血液制品辐照是预防输血后移植物抗宿主病（PT-GVHD）的有效方法。我们借助³H-胸腺嘧啶核苷摄取，检测了不同剂量X射线辐照后血液制品中淋巴细胞的有丝分裂活性。结果显示，仅5 Gy的辐照就能完全消除混合淋巴细胞培养（MLC）反应。50 Gy的辐照完全消除了丝裂原刺激的³H-胸腺嘧啶核苷摄取（丝裂原反应）。超过15 Gy的辐照可消除PT-GVHD风险，因为MLC反应似乎与患者PT-GVHD的机制密切相关。此外，研究表明，高达50 Gy的X射线辐照对血液制品的体外特性无有害影响。然而，我们必须注意辐照后储存的血液制品血浆中钾的高浓度。
