Parra O M, Henandez-Blasquez F J, da Silva J R, Sousa Silva R A, Saad W A, Saad Júnior W A
University of São Paulo Medical School, Brasil.
Arq Gastroenterol. 1996 Oct-Dec;33(4):212-6.
During regeneration after partial hepatectomy, the hepatic parenchyma grows by reproducing its histological elements at different rates. The hepatic cells grow more rapidly and the collagen components of the extracellular matrix more slowly. This report studies the behavior of liver tissue collagen in intact livers, increased in size by stimulation with exogenous hepatotrophic factors, comparing them to regenerated livers after hepatectomy. The series consists of a group of rats (n = 4), seven days after 70% hepatectomy, with an average growth of the residual liver mass of 71.55% and another group (n = 4), seven days after stimulation of the intact livers with an average growth of 121.05%. The exogenous hepatotrophic factors administered intraperitoneally (portal) included glucose, amino acids, insulin, glucagon and triiodothyronine. The hepatic collagen content was compared by studying slides stained with Picrossirius and examined with an optical microscope attached to a computer with an image analyzing program. The hepatic collagen was reduced from 2.07% +/- 0.13 to 1.14 +/- 0.08 (a difference of 44.57%; P < 0.001) after hepatectomy and from 1.98% +/- 0.19 to 1.24% +/- 0.07 (difference of 37.46%; P < 0.01) after exogenous hepatotrophic factors stimulation. It was concluded that the production of collagen in hepatic growth initiating with intact livers stimulated by exogenous hepatotrophic factors, occurs with a time difference similarly to what is observed after 70% hepatectomy.
在部分肝切除术后的再生过程中，肝实质通过以不同速率复制其组织学成分而生长。肝细胞生长较快，而细胞外基质的胶原成分生长较慢。本报告研究了在完整肝脏中，经外源性肝营养因子刺激体积增大后的肝组织胶原行为，并将其与肝切除术后的再生肝脏进行比较。该系列包括一组大鼠（n = 4），在70%肝切除术后7天，残余肝脏质量平均增长71.55%，另一组（n = 4），在完整肝脏经刺激后7天，平均增长121.05%。经腹腔（门静脉）给予的外源性肝营养因子包括葡萄糖、氨基酸、胰岛素、胰高血糖素和三碘甲状腺原氨酸。通过研究用天狼星红染色的切片，并使用连接有图像分析程序的计算机的光学显微镜进行检查，比较肝脏胶原含量。肝切除术后肝脏胶原含量从2.07%±0.13降至1.14±0.08（差异为44.57%；P < 0.001），在外源性肝营养因子刺激后从1.98%±0.19降至1.24%±0.07（差异为37.46%；P < 0.01）。得出的结论是，在外源性肝营养因子刺激的完整肝脏启动的肝脏生长过程中，胶原的产生存在时间差异，这与70%肝切除术后观察到的情况类似。
