Yamamoto H, Murawaki Y, Kawasaki H
Second Department of Internal Medicine, Tottori University School of Medicine, Yonago, Japan.
Hepatology. 1995 Jan;21(1):155-61.
To elucidate hepatic collagen metabolism during liver regeneration after partial hepatectomy, we measured collagen content, collagen synthesis, and collagen-degrading enzyme activity in the remnant livers of rats 3, 5, 7, and 14 days after a partial hepatectomy of 68%. Hepatic collagen synthesis was significantly higher 3, 5, and 7 days after partial hepatectomy than it was in sham-operated control rats, but there was no such difference 14 days after surgery, the maximal hepatic collagen synthesis being observed 5 days after surgery. Although the collagen concentration in the remnant liver was similar to that in the control liver, the total collagen content of the remnant liver increased rapidly with liver regeneration until 7 days after partial hepatectomy. Hepatic collagenase activity was similar to the control; however, hepatic cathepsin B and cathepsin L activity and the intracellular degradation of newly synthesized collagen were markedly decreased 3, 5, and 7 days after partial hepatectomy compared with the controls. Hepatic collagen synthesis was significantly and inversely correlated with cathepsin L activity and with the intracellular degradation of newly synthesized collagen. These findings suggest that a combination of increased collagen synthesis and decreased intracellular collagen degradation contributes to the rapid supply of collagen that is observed during the early phase of liver regeneration.
为阐明部分肝切除术后肝脏再生过程中的肝胶原代谢，我们在大鼠进行68%部分肝切除术后3、5、7和14天，测量了残余肝脏中的胶原含量、胶原合成及胶原降解酶活性。部分肝切除术后3、5和7天，肝胶原合成显著高于假手术对照大鼠，但术后14天无此差异，术后5天观察到最大肝胶原合成。尽管残余肝脏中的胶原浓度与对照肝脏相似，但直到部分肝切除术后7天，残余肝脏的总胶原含量随肝脏再生而迅速增加。肝胶原酶活性与对照相似；然而，与对照相比，部分肝切除术后3、5和7天，肝组织蛋白酶B和组织蛋白酶L活性以及新合成胶原的细胞内降解明显降低。肝胶原合成与组织蛋白酶L活性以及新合成胶原的细胞内降解显著负相关。这些发现表明，胶原合成增加与细胞内胶原降解减少共同作用，促成了肝脏再生早期所观察到的胶原快速供应。
