Liu S H, Al-Shaikh R A, Panossian V, Finerman G A, Lane J M
Department of Orthopaedic Surgery, UCLA School of Medicine 90095, USA.
Am J Sports Med. 1997 Sep-Oct;25(5):704-9. doi: 10.1177/036354659702500521.
Investigations from this laboratory have established the presence of estrogen receptors in the human anterior cruciate ligament. This study further investigates the effects of 17 beta-estradiol on the cellular proliferation and collagen synthesis of fibroblasts derived from the rabbit anterior cruciate ligament. Fibroblast proliferation and collagen synthesis in response to near log concentrations of 17 beta-estradiol (at 0.0029, 0.025, 0.25, 2.5, and 25 ng/ml) were assessed by measuring [3H]thymidine and [14C]hydroxyproline incorporation, respectively. Collagen synthesis was significantly reduced with increasing local estradiol concentration (P < 0.001). Declining collagen synthesis was first noted at a 17 beta-estradiol concentration of 0.025 ng/ml. Within normal physiologic levels of estrogen (0.025 to 0.25 ng/ml), collagen synthesis was reduced by more than 40% of control, and at pharmacologic levels of 2.5 and 25 ng/ml, by more than 50% of control. A significant reduction of fibroblast proliferation was also observed with increasing estradiol concentrations (P = 0.023). Clinically, alterations in anterior cruciate ligament cellular metabolism caused by estrogen fluctuations may change the composition of the ligament, rendering it more susceptible to injury.
本实验室的研究已证实人类前交叉韧带中存在雌激素受体。本研究进一步探讨了17β-雌二醇对兔前交叉韧带成纤维细胞的细胞增殖和胶原蛋白合成的影响。通过分别测量[3H]胸腺嘧啶核苷和[14C]羟脯氨酸掺入量，评估了成纤维细胞对接近对数浓度的17β-雌二醇（0.0029、0.025、0.25、2.5和25 ng/ml）的增殖和胶原蛋白合成情况。随着局部雌二醇浓度的增加，胶原蛋白合成显著减少（P < 0.001）。在17β-雌二醇浓度为0.025 ng/ml时首次发现胶原蛋白合成下降。在雌激素的正常生理水平（0.025至0.25 ng/ml）范围内，胶原蛋白合成比对照组减少了40%以上，而在2.5和2５ ng/ml的药理水平下，比对照组减少了50%以上。随着雌二醇浓度的增加，成纤维细胞增殖也显著减少（P = 0.023）。临床上，雌激素波动引起的前交叉韧带细胞代谢改变可能会改变韧带的组成，使其更容易受伤。
