Tóth I E, Palkovits M
Joint Research Laboratory of Neuromorphology, Semmelweis University Medical School, Budapest, Hungary.
Neurobiology (Bp). 1997;5(1):17-41.
A method has recently been developed to study the neuroanatomical connections in the brain by trans-synaptic tract-tracing via neurotropic viruses. Neurotrop viruses injected into a peripheral organ or directly into the central nervous system are transported axonally. Viruses are expressed in the infected neurons and they are transferred through synapses to reach other neurons. Many research studies illustrate by immunocytochemical detection of the viral proteins that the trans-synaptically interconnected neurons can be visualized, in addition, their neurochemical character can be identified. Thus, viruses could serve as a self-amplifying specific markers of connected neurons along hierarchial chains of functionally related circuits. Herein, we reviewed the methodology of the neuroanatomical studies obtained with a member of a-herpes viruses, the pseudorabies virus, frequently used in tracer studies in rats.
最近开发了一种通过嗜神经病毒进行跨突触束路追踪来研究大脑神经解剖连接的方法。注入外周器官或直接注入中枢神经系统的嗜神经病毒通过轴突运输。病毒在受感染的神经元中表达，并通过突触转移到其他神经元。许多研究通过对病毒蛋白的免疫细胞化学检测表明，跨突触相互连接的神经元可以可视化，此外，还可以确定它们的神经化学特征。因此，病毒可以作为沿着功能相关回路的层次链连接的神经元的自我放大特异性标记。在此，我们回顾了用α疱疹病毒成员伪狂犬病病毒进行神经解剖学研究的方法，该病毒常用于大鼠的示踪研究。
