Raaphorst G P, Mao J P, Yang D P, Ng C E
Department of Medical Physics, Ottawa Regional Cancer Centre, Ontario, Canada.
Radiat Oncol Investig. 1997;5(1):1-7. doi: 10.1002/(sici)1520-6823(1997)5:1<1::aid-roi1>3.0.co;2-1.
Two human melanoma cell lines (one radiosensitive, HT144 and one radioresistant, SK Mel-3) and one normal human fibroblast (AG1522) were evaluated for thermal radiosensitization and the thermal enhancement ratios (TERs) were calculated. These were compared with residual polymerase activity to determine if this activity could be used to predict TERs. In all three cell lines, there was a good correlation between TER and residual polymerase alpha or beta activity. Polymerase beta was more sensitive than polymerase alpha as an indicator for TER. There were small cell line-dependent differences (not related to radiosensitivity) among the correlation curves, indicating that for each cell/tumor-type polymerase activity, vs. TER may have to be calibrated.
对两个人类黑色素瘤细胞系（一个对辐射敏感，HT144；一个对辐射抵抗，SK Mel-3）和一个正常人成纤维细胞（AG1522）进行了热辐射增敏评估，并计算了热增强比（TERs）。将这些结果与残留聚合酶活性进行比较，以确定该活性是否可用于预测TERs。在所有三个细胞系中，TER与残留的聚合酶α或β活性之间存在良好的相关性。作为TER的指标，聚合酶β比聚合酶α更敏感。在相关曲线之间存在与细胞系相关的微小差异（与辐射敏感性无关），这表明对于每种细胞/肿瘤类型，可能都需要校准聚合酶活性与TER之间的关系。
