Westphal J R, van't Hullenaar R G, van der Laak J A, Cornelissen I M, Schalkwijk L J, van Muijen G N, Wesseling P, de Wilde P C, Ruiter D J, de Waal R M
Department of Pathology, University Hospital Nijmegen, The Netherlands.
Br J Cancer. 1997;76(5):561-70. doi: 10.1038/bjc.1997.427.
We studied the relation between tumour vascular density and tumour growth rate, metastatic incidence and vascular permeability factor (VPF) mRNA levels in a human xenograft model described previously. Vascular density was determined by automated image analysis. Xenografts derived from cell lines BLM and MV3 showed the highest mean vascular density (MVD), the highest in vivo growth rate, high VPF mRNA levels and rapid development of lung metastases. Xenografts of cell lines M14, Mel57 and MV1 showed a significantly lower degree of vascularization, lower in vivo growth rates and lower levels of VPF mRNA, but formed lung metastases with a similar incidence as those of BLM and MV3. Xenografts from cell line 1F6 did not form lung metastases, whereas tumours derived from a spontaneous mutant of 1F6, designated 1F6m, gave rise to lung metastases to the same extent as Mel57, M14 and MV1 tumours. MVD values in 1F6 and 1F6m xenografts, VPF mRNA levels and in vivo growth rates of 1F6 and 1 F6m xenografts, however, were similar. In conclusion, in the melanoma xenograft model vascular density is correlated with in vivo growth rate and with in vitro VPF mRNA levels, but not with the ability to metastasize.
我们在先前描述的人异种移植模型中研究了肿瘤血管密度与肿瘤生长速率、转移发生率以及血管通透因子（VPF）mRNA水平之间的关系。血管密度通过自动图像分析来确定。源自细胞系BLM和MV3的异种移植瘤显示出最高的平均血管密度（MVD）、最高的体内生长速率、较高的VPF mRNA水平以及肺转移的快速发展。细胞系M14、Mel57和MV1的异种移植瘤血管化程度显著较低，体内生长速率较低，VPF mRNA水平也较低，但形成肺转移的发生率与BLM和MV3的相似。细胞系1F6的异种移植瘤未形成肺转移，而源自1F6自发突变体（命名为1F6m）的肿瘤产生肺转移的程度与Mel57、M14和MV1肿瘤相同。然而，1F6和1F6m异种移植瘤的MVD值、VPF mRNA水平以及1F6和1F6m异种移植瘤的体内生长速率相似。总之，在黑色素瘤异种移植模型中，血管密度与体内生长速率以及体外VPF mRNA水平相关，但与转移能力无关。
