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[从不同年龄动物的小肠肠上皮细胞中分离质膜组分]

[The isolation of plasma membrane fractions from the enterocytes of the small intestine in animals of different ages].

作者信息

Tsvilikhovs'kyĭ M I

出版信息

Fiziol Zh (1994). 1997;43(3-4):101-5.

PMID:9303792
Abstract

The described methodical conditions of obtaining apical and basolateral membranes from the suspension of isolated small intestine epithelial cells of cattle, that are more effective, in comparison with mucous membrane scrapings by certain criteria (material output, purity level, presence of pollution, vesiculosity and orientation of vesicles). It is determined, that apical and basolateral membranes of newborn animals have their specific features as to the amount of protein, pollution, purification in comparison with the mature animals. For several biochemical features the fractions of apical and basolateral membranes of enterocytes of the small intestine in mature and newborn animals might be recommended for the investigations of different biochemical processes, including the norm and pathology of the digestive tract.

摘要

所述从牛分离的小肠上皮细胞悬液中获取顶端膜和基底外侧膜的方法条件,与通过某些标准(材料产量、纯度水平、污染情况、囊泡化程度和囊泡取向)进行的粘膜刮取相比,更有效。已确定,与成熟动物相比,新生动物的顶端膜和基底外侧膜在蛋白质含量、污染情况、纯化方面具有其特定特征。对于几种生化特征,成熟和新生动物小肠肠上皮细胞的顶端膜和基底外侧膜组分可能推荐用于不同生化过程的研究,包括消化道的正常和病理情况。

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