Saitta A, Saitta M N, Messina A, Bonaiuto M, Cinquegrani M, Squadrito G, Castaldo M, Sardo A, Imbalzano E, Squadrito F
Dipartimento di Medicina Interna e Terapia Medica, Università degli Studi, Messina.
Minerva Med. 1997 Jul-Aug;88(7-8):275-82.
It has been studied whether an adrenergic stimulation induced by the cold pressor test (CPT) could influence the behaviour of the transmembrane transport systems of sodium in hypertensive subjects compared to a normotensive control population.
Twenty-two hypertensive subjects (average age 43.2 +/- 5.7 years), with normal weight, without signs of cardiovascular and metabolic diseases, underwent the cold pressor test. The dynamic behaviour of sodium erythrocytic transport systems and plasmatic norepinephrine was evaluated basally, at the third minute during the cold pressor test and 20 minutes after the end of the test. The same test was carried out in a control population made up of 20 normotensive subjects (average age 41.9 +/- 4.8 years), selected on the basis of the absence of any cardiovascular or metabolic pathology and without family history of arterial hypertension.
The cold pressor test did not cause significant changes in the sodium transmembrane transport systems in normotensive subjects, while in the hypertensive subjects a significant reduction was observed, during the test, in the total efflux of sodium and in the sodium/potassium pump, respectively from 2636 +/- 296 mumol/l/red blood cells/hr to 2032 +/- 178 mumol/l/red blood cells/hr (p < 0.0001) and from 2156 +/- 149 mumol/l/red blood cells/hr to 1610 +/- 101 mumol/l/red blood cells/hr (p < 0.0001); the intraerythrocytic sodium increased from 6.5 +/- 1.0 mmol/l/cells to 7.2 +/- 1.1 mmol/l/cells (p < 0.04) and the passive permeability decreased from 0.039 +/- 0.004 hr-1 to 0.018 +/- 0.006 hr-1 (p < 0.0001). During cold pressor test the increase in the plasma norepinephrine levels was correlated to the reduction in the total efflux of sodium (r = -0.60; p < 0.003) and in the sodium/potassium pump (r = -0.59; p < 0.003) only in hypertensive subjects.
Our data show that an adrenergic stimulation, induced by the cold pressor test, is able to significantly influence the behaviour of transmembrane fluxes of sodium in hypertensive subjects, and it causes an inhibitory effect on the sodium/potassium pump and an increase in the intraerythrocytic sodium. Such data show the existence in hypertensive subjects of an interrelationship between adrenergic activity and sodium transport systems that could cooperate in causing and/or in maintaining the hypertensive syndrome.
研究了冷加压试验（CPT）诱导的肾上腺素能刺激与正常血压对照人群相比，是否会影响高血压患者钠跨膜转运系统的行为。
22名体重正常、无心血管和代谢疾病迹象的高血压患者（平均年龄43.2±5.7岁）接受了冷加压试验。在冷加压试验前、试验第3分钟和试验结束后20分钟，评估了红细胞钠转运系统和血浆去甲肾上腺素的动态行为。在由20名正常血压受试者组成的对照人群中进行了相同的试验，这些受试者基于无任何心血管或代谢病理且无动脉高血压家族史进行选择（平均年龄41.9±4.8岁）。
冷加压试验在正常血压受试者中未引起钠跨膜转运系统的显著变化，而在高血压受试者中，试验期间观察到钠总流出量和钠/钾泵分别从2636±296μmol/l/红细胞/小时显著降低至2032±178μmol/l/红细胞/小时（p<0.0001）和从2156±149μmol/l/红细胞/小时降至1610±101μmol/l/红细胞/小时（p<0.0001）；红细胞内钠从6.5±1.0mmol/l/细胞增加至7.2±1.1mmol/l/细胞（p<0.04），被动通透性从0.039±0.004小时-1降至0.018±0.006小时-1（p<0.0001）。在冷加压试验期间，仅在高血压受试者中，血浆去甲肾上腺素水平的升高与钠总流出量的降低（r=-0.60；p<0.003）和钠/钾泵的降低（r=-0.59；p<0.003）相关。
我们的数据表明，冷加压试验诱导的肾上腺素能刺激能够显著影响高血压患者钠跨膜通量的行为，并对钠/钾泵产生抑制作用，导致红细胞内钠增加。这些数据表明高血压患者中肾上腺素能活性与钠转运系统之间存在相互关系，这可能在导致和/或维持高血压综合征中起协同作用。
