人视网膜母细胞瘤细胞系中葡萄糖转运蛋白异构体表达的差异。

Differences in the expression of glucose transporter protein isoforms in human retinoblastoma cell lines.

作者信息

Tsukamoto H, Mishima H, Hirata K, Sato E, Kurokawa T, Ishibashi S

机构信息

Department of Ophthalmology, Hiroshima University School of Medicine, Japan.

出版信息

Jpn J Ophthalmol. 1997 Jul-Aug;41(4):226-30. doi: 10.1016/s0021-5155(97)00041-5.

DOI:10.1016/s0021-5155(97)00041-5

PMID:9304435

Abstract

We investigated the expression of glucose transporter (GLUT) protein isoforms in two human retinoblastoma cell lines, Y79 and WERI-Rb1, by Western blotting analysis with anti-GLUT1, 2, 3, and 4 antibodies. GLUT1 and GLUT4 proteins were detected in Y79, whereas GLUT1 and GLUT3 proteins were found in WERI-Rb1. GLUT2 protein was not detected in Y79 or WERI-Rb1. Our findings are of interest because (1) the expression of GLUT protein isoforms in the two retinoblastoma cell lines was different, and (2) GLUT4 protein, the insulin-sensitive GLUT isoform, was detected in Y79. This suggests that these cell lines have different mechanisms of glucose transport.

摘要

我们使用抗葡萄糖转运蛋白（GLUT）1、2、3和4抗体，通过蛋白质免疫印迹分析，研究了两种人视网膜母细胞瘤细胞系Y79和WERI-Rb1中葡萄糖转运蛋白（GLUT）蛋白亚型的表达情况。在Y79细胞系中检测到了GLUT1和GLUT4蛋白，而在WERI-Rb1细胞系中发现了GLUT1和GLUT3蛋白。在Y79或WERI-Rb1细胞系中均未检测到GLUT2蛋白。我们的研究结果具有重要意义，原因如下：（1）两种视网膜母细胞瘤细胞系中GLUT蛋白亚型的表达存在差异；（2）在Y79细胞系中检测到了胰岛素敏感的GLUT亚型GLUT4蛋白。这表明这些细胞系具有不同的葡萄糖转运机制。