Allen T D, Bennion G R, Rutherpord S A, Reipert S, Ramalho A, Kiseleva E, Goldberg M W
CRC Department of Structural Cell Biology, Paterson Institute for Cancer Research, Christie Hospital NHS Trust, Manchester, U.K.
Scanning. 1997 Sep;19(6):403-10. doi: 10.1002/sca.4950190603.
Scanning electron microscopy (SEM) has produced a wealth of novel images that have significantly complemented our perception of biological structure and function, derived initially from transmission electron microscopy (TEM) information. SEM is a surface imaging technology, and its impact at the subcellular level has been restricted by reduced resolution in comparison with TEM. Recently, SEM resolution has been considerably improved by the advent of high-brightness sources used in field-emission instruments (FEISEM) which have produced resolution of around 1 nm, virtually equivalent to TEM "working resolution." Here we review our findings in the use of FEISEM in the imaging of nuclear envelopes and their associated structures, such as nuclear pore complexes, and the relationships of structure and function. FEISEM allows the structurally orientated cell biologist to visualise, directly and in three dimensions, subcellular structure and its modulation with a view to understanding its functional significance.
扫描电子显微镜（SEM）产生了大量新颖的图像，这些图像极大地补充了我们最初从透射电子显微镜（TEM）信息中获得的对生物结构和功能的认识。SEM是一种表面成像技术，与TEM相比，其在亚细胞水平上的影响因分辨率降低而受到限制。最近，场发射仪器（FEISEM）中使用的高亮度源的出现使SEM分辨率有了显著提高，产生了约1纳米的分辨率，几乎等同于TEM的“工作分辨率”。在此，我们回顾了在使用FEISEM对核膜及其相关结构（如核孔复合体）进行成像以及结构与功能关系方面的研究结果。FEISEM使结构导向的细胞生物学家能够直接以三维方式可视化亚细胞结构及其调节，从而理解其功能意义。
