海洋贻贝中蛋白激酶C亚型的鉴定

Identification of protein kinase C isoforms in marine mussels.

作者信息

Barcia R, Lopez-Garcia J M, Girardini J E, Ramos-Martinez J I

机构信息

Departamento de Bioquímica y Biología Molecular, Universidad de Santiago de Compostela, Facultad de Veterinaria, Lugo, Spain.

出版信息

Biochem Mol Biol Int. 1997 Sep;42(6):1241-8. doi: 10.1080/15216549700203711.

DOI:10.1080/15216549700203711

PMID:9305542

Abstract

In this paper we have examined the distribution of some isoforms of protein kinase C in different tissues from the sea mussel Mytilus galloprovincialis Lmk.. By immunoblot analysis, we have detected the presence of at least three PKC isoforms, all preferably associated with the cellular cytosolic fraction. The Ca(2+)-independent form PKC delta was separated from the Ca(2+)-dependent forms (PKC alpha and beta) by means of an ionic change chromatography on DE-52. A comparative study was carried out on the phosphorylatable non-artificial substrates. The M.B.P. protein proved to be the best substrate, while the worst was HIIIS histone which, however, is frequently used in evaluation assays of the activity.

摘要

在本文中，我们研究了贻贝（Mytilus galloprovincialis Lmk.）不同组织中蛋白激酶C某些同工型的分布。通过免疫印迹分析，我们检测到至少三种蛋白激酶C同工型的存在，它们都优先与细胞胞质部分相关。通过在DE-52上进行离子交换色谱，将不依赖钙的蛋白激酶Cδ同工型与依赖钙的同工型（蛋白激酶Cα和β）分离。对可磷酸化的非人工底物进行了比较研究。髓鞘碱性蛋白（M.B.P.）被证明是最佳底物，而最差的是HIIIS组蛋白，不过它在活性评估试验中经常被使用。