Lurton D, Cavalheiro E A
Neurologia Experimental, Escola Paulista de Medicina-UNIFESP, São Paulo, Brazil.
Exp Brain Res. 1997 Aug;116(1):186-90. doi: 10.1007/pl00005739.
Neuropeptide-Y (NPY) is expressed by granule cells and mossy fibres of the hippocampal dentate gyrus during experimental temporal lobe epilepsy (TLE). This expression may represent an endogenous damping mechanism since NPY has been shown to block seizure-like events following high-frequency stimulation in hippocampal slices. The pilocarpine (PILO) model of epilepsy is characterized by an acute period of status epilepticus followed by spontaneous recurrent seizures and related brain damage. We report peroxidase-antiperoxidase immunostaining for NPY in several brain regions in this model. PILO-injected animals exhibited NPY immunoreactivity in the region of the mossy fibre terminals, in the dentate gyrus inner molecular layer and, in a few cases, within presumed granule cells. NPY immunoreactivity was also dramatically changed in the entorhinal cortex, amygdala and sensorimotor areas. In addition, PILO injected animals exhibited a reduction in the number of NPY-immunoreactive interneurons compared with controls. The results demonstrate that changes in NPY expression, including expression in the granule cells and mossy fibres and the loss of vulnerable NPY neurons, are present in the PILO model of TLE. However, the significance of this changed synthesis of NPY remains to be determined.
在实验性颞叶癫痫（TLE）期间，海马齿状回的颗粒细胞和苔藓纤维表达神经肽Y（NPY）。这种表达可能代表一种内源性抑制机制，因为在海马切片中高频刺激后，NPY已被证明可阻断癫痫样事件。癫痫的毛果芸香碱（PILO）模型的特征是癫痫持续状态的急性期，随后是自发性反复癫痫发作和相关脑损伤。我们报告了该模型中几个脑区NPY的过氧化物酶-抗过氧化物酶免疫染色。注射PILO的动物在苔藓纤维终末区域、齿状回内分子层以及少数情况下在假定的颗粒细胞内表现出NPY免疫反应性。内嗅皮质、杏仁核和感觉运动区域的NPY免疫反应性也发生了显著变化。此外，与对照组相比，注射PILO的动物NPY免疫反应性中间神经元数量减少。结果表明，TLE的PILO模型中存在NPY表达的变化，包括颗粒细胞和苔藓纤维中的表达以及易损NPY神经元的丢失。然而，NPY这种合成变化的意义仍有待确定。
