King-Herbert A P, Hesterburg T W, Thevenaz P P, Hamm T E, Moss O R, Janszen D B, Everitt J I
Chemical Industry Institute of Toxicology, Research Triangle Park, North Carolina, USA.
Lab Anim Sci. 1997 Aug;47(4):362-6.
Rodent nose-only inhalation toxicology systems comprise whole-body immobilization in plastic restraint tubes. This method of restraint is known to have a variety of effects on animals. In the studies reported here, two independent toxicology laboratories examined the effects of inhalation tube restraint in Syrian golden hamsters, a species that has recently gained importance in inhalation studies of fibrous particulates. Body weight, food and water consumption, core body temperature, and plasma cortisol and corticosterone concentrations were assessed in animals immobilized in nose-only inhalation tubes, and the results were compared with those from unrestrained cage-control animals. Animals were immobilized for either 6 h/ day, 5 days/week for 13 weeks (subchronic), or 4 h/day for 14 consecutive days (subacute), mimicking exposure conditions commonly used in nose-only inhalation studies. Tube restraint was found to induce a marked decrease in body weight, which increased in response to cessation of restraint. The body weight decrement was associated with significant differences in food and water consumption between the restrained and control groups in the subacute study and only food consumption in the subchronic study. During the restraint period, core body temperature in the immobilized animals increased slightly but not above the normal range for this species. Plasma cortisol and corticosterone concentrations were not significantly increased with use of restraint, compared with values in controls. Immobilization-associated body weight depression in Syrian golden hamsters is important for the evaluation of nose-only inhalation study results because many normal physiologic parameters, as well as toxicant-induced effects, are associated with body weight status.
啮齿动物仅鼻吸入毒理学系统包括将动物全身固定在塑料约束管中。已知这种约束方法会对动物产生多种影响。在本文报道的研究中，两个独立的毒理学实验室研究了吸入管约束对叙利亚金黄地鼠的影响，该物种最近在纤维状颗粒吸入研究中变得越来越重要。对固定在仅鼻吸入管中的动物评估体重、食物和水的消耗量、核心体温以及血浆皮质醇和皮质酮浓度，并将结果与未受约束的笼养对照动物的结果进行比较。动物被固定6小时/天，每周5天，持续13周（亚慢性），或连续14天每天固定4小时（亚急性），模拟仅鼻吸入研究中常用的暴露条件。发现管约束会导致体重显著下降，在约束停止后体重会增加。在亚急性研究中，体重下降与受约束组和对照组之间食物和水消耗量的显著差异有关，而在亚慢性研究中仅与食物消耗量有关。在约束期间，固定动物的核心体温略有升高，但未超过该物种的正常范围。与对照组相比，使用约束时血浆皮质醇和皮质酮浓度没有显著增加。叙利亚金黄地鼠中与固定相关的体重下降对于评估仅鼻吸入研究结果很重要，因为许多正常生理参数以及毒物诱导的效应都与体重状况有关。
