Lamerigts N, Aspenberg P, Buma P, Versleyen D, Slooff T J
Department of Orthopaedics, University Hospital Nijmegen, The Netherlands.
Lab Anim Sci. 1997 Aug;47(4):401-6.
We developed a repeated sampling bone chamber (RSBC) and tested its suitability for studying various aspects of the bone allograft incorporation process under reproducible nonload-bearing experimental conditions in a large vertebrate. Our chamber is made of commercially pure titanium and is designed to allow bone or tissue ingrowth into a removable hollow inner core. Three chambers per animal were randomly implanted in the tibias of 10 goats and were harvested every 8 weeks. In experiment 1, two chambers were filled with a fresh-frozen structural allograft or a chip allograft, and one was left empty. In experiment 2, all chambers were left empty to measure intra- and interanimal variation. The results were evaluated by histomorphometry. Clinical results of four growth factor experiments also are presented. Using this model, we conducted 60 harvest operations (median, 4/animal; range, 2 to 8). In experiment 1, more soft tissue ingrowth and osteoclasts were measured in the chambers with allograft (P < 0.005 and P < 0.03 respectively). Bone ingrowth was scant, with no significant differences between chip graft, structural graft, and empty control chamber. Thus, the bone graft did not show any osteoinductive or osteoconductive properties. Experiment 2 indicated consistent tissue ingrowth, with greater interanimal variation than variations among the chambers in any goat. Our method forms a means of studying gradual tissue and bone ingrowth into bone grafts. The inherent low amount of bone ingrowth makes this model suitable for studying bone-inductive substances. Repeated sampling in the same animals lowered the intersample variability and reduced the number of animals that were required.
我们研发了一种重复采样骨腔（RSBC），并在大型脊椎动物可重复的非负重实验条件下，测试了其在研究同种异体骨植入过程各个方面的适用性。我们的骨腔由商业纯钛制成，设计目的是允许骨或组织向内生长到一个可移除的中空内芯中。每只动物随机在其胫骨中植入三个骨腔，每8周进行一次采集。在实验1中，两个骨腔填充新鲜冷冻的结构性同种异体骨或碎骨同种异体骨，一个骨腔为空。在实验2中，所有骨腔均为空，以测量动物体内和动物间的差异。通过组织形态计量学对结果进行评估。还展示了四个生长因子实验的临床结果。使用该模型，我们进行了60次采集操作（中位数为每只动物4次；范围为2至8次）。在实验1中，与同种异体骨填充的骨腔相比，测量到更多的软组织向内生长和破骨细胞（分别为P < 0.005和P < 0.03）。骨向内生长较少，碎骨移植、结构性移植和空对照骨腔之间无显著差异。因此，骨移植未显示出任何骨诱导或骨传导特性。实验2表明组织向内生长一致，动物间差异大于任何一只山羊体内不同骨腔之间的差异。我们的方法形成了一种研究组织和骨逐渐向内生长到骨移植中的手段。骨向内生长量低这一固有特点使该模型适用于研究骨诱导物质。在同一动物中重复采样降低了样本间的变异性，并减少了所需动物的数量。
