Gerloni M, Baliou W R, Billetta R, Zanetti M
Department of Medicine, University of California, San Diego 92093-0063, USA.
Nat Biotechnol. 1997 Sep;15(9):876-81. doi: 10.1038/nbt0997-876.
Immunity against the human malaria parasite Plasmodium falciparum was induced using somatic transgene immunization, a method to effectively target B lymphocytes in vivo. A single inoculation of plasmid DNA containing an immunoglobulin heavy-chain gene coding in the complementarity-determining region 3 for three repeats of the sequence Asn-Ala-Asn-Pro (NANP), a B-cell epitope of P.falciparum sporozoites, induced antibodies against NANP in all mice. A booster with an antibody antigenized with the NANP peptide, or challenge with P. falciparum sporozoites, demonstrated the establishment of immunologic memory. Immunity to a parasite antigen can be induced by exploiting mechanisms in which B lymphocytes are both the source of the immunogen as well as the effector mechanism of immunity. The results indicate that somatic transgene immunization is a potential approach for vaccination against foreign pathogens.
利用体细胞转基因免疫诱导针对人类疟原虫恶性疟原虫的免疫力，这是一种在体内有效靶向B淋巴细胞的方法。单次接种含有免疫球蛋白重链基因的质粒DNA，该基因在互补决定区3编码疟原虫子孢子的B细胞表位Asn-Ala-Asn-Pro（NANP）序列的三个重复，可在所有小鼠中诱导出针对NANP的抗体。用NANP肽抗原化的抗体进行加强免疫，或用恶性疟原虫子孢子进行攻击，证明了免疫记忆的建立。通过利用B淋巴细胞既是免疫原来源又是免疫效应机制的机制，可以诱导对寄生虫抗原的免疫。结果表明，体细胞转基因免疫是针对外来病原体进行疫苗接种的一种潜在方法。
