Takahashi T, Kawashima M, Yasuoka T, Tanaka K
Department of Biological Diversity and Resources, Gifu University, Japan.
J Reprod Fertil. 1997 Jul;110(2):245-8. doi: 10.1530/jrf.0.1100245.
The molecular mass of the arginine vasotocin receptor in membrane fractions of the uterus (shell gland) of hens was estimated by autoradiography of [125I]-labelled arginine vasotocin binding protein after SDS-PAGE. A band of approximately 67 kDa was found in all of the samples taken from laying hens before and after oviposition and from nonlaying hens. In addition to this band, another band of approximately 95 kDa was found in samples from laying hens taken immediately before and after oviposition. Both bands were reduced by the presence of unlabelled arginine vasotocin or mesotocin, but the reduction was greater by arginine vasotocin than by mesotocin. These two bands were not reduced by chicken luteinizing hormone-releasing hormone-I (Gln8-GnRH), chicken luteinizing hormone-releasing hormone-II (His5,Trp7,Tyr8-GnRH) and chicken angiotensin-II (Val5-angiotensin-II). The appearance of the arginine vasotocin receptor of the larger molecular size may be related to oviposition in hens.
通过十二烷基硫酸钠 - 聚丙烯酰胺凝胶电泳（SDS - PAGE）后对[125I]标记的精氨酸加压催产素结合蛋白进行放射自显影，估算了母鸡子宫（壳腺）膜组分中精氨酸加压催产素受体的分子量。在取自产蛋前后的产蛋母鸡以及非产蛋母鸡的所有样品中均发现了一条约67 kDa的条带。除了这条带之外，在紧接产蛋前后采集的产蛋母鸡样品中还发现了另一条约95 kDa的条带。未标记的精氨酸加压催产素或中催产素的存在会使这两条带减弱，但精氨酸加压催产素引起的减弱比中催产素更大。鸡促黄体生成素释放激素 - I（Gln8 - GnRH）、鸡促黄体生成素释放激素 - II（His5，Trp7，Tyr8 - GnRH）和鸡血管紧张素 - II（Val5 - 血管紧张素 - II）不会使这两条带减弱。较大分子大小的精氨酸加压催产素受体的出现可能与母鸡产蛋有关。
