Wolff R L, Christie W W, Coakley D
ISTAB, Laboratoire de Lipochimie Alimentaire, Université Bordeaux, Talence, France.
Lipids. 1997 Sep;32(9):971-3. doi: 10.1007/s11745-997-0126-9.
14-Methylhexadecanoic (14-MHD) acid has been identified in a sample of pine seed oil (Pinus contorta) by gas-liquid chromatography-mass spectrometry of its picolinyl ester derivative. Its identification (through its equivalent chain length) and its distribution in four conifer families have been checked. It occurred only in Pinaceae, where it was found in 72 species belonging to the genera Pinus, Abies, Cedrus, Tsuga, Pseudotsuga, Larix, and Picea, in the range 0.02-1.15%. 14-MHD acid could not be detected in the lipids of Taxaceae (Taxus baccata), Cupressaceae (Juniperus communis), or Taxodiaceae (Sciadopytis verticillata), even after a 10-fold concentration of the saturated acid fraction isolated by argentation thin-layer chromatography. It is concluded that Pinaceae, along with Ginkgo biloba seed lipids, are major exceptions in the plant kingdom with regard to 14-MHD acid, which otherwise occurs almost exclusively in lipids of animals and microorganisms. The biosynthesis and metabolic role of 14-MHD acid, which otherwise also occur in wood and leaf lipids, remain unknown.
通过对其吡啶甲酯衍生物进行气液色谱 - 质谱分析，在扭叶松种子油样本中鉴定出了14 - 甲基十六烷酸（14 - MHD）。已核查了其鉴定结果（通过其等效链长）及其在四个针叶树科中的分布情况。它仅存在于松科中，在松属、冷杉属、雪松属、铁杉属、黄杉属、落叶松属和云杉属的72个物种中被发现，含量范围为0.02 - 1.15%。即使对通过银化薄层色谱分离得到的饱和酸馏分进行了10倍浓缩，在红豆杉科（欧洲红豆杉）、柏科（刺柏）或杉科（日本金松）的脂质中仍未检测到14 - MHD酸。得出的结论是，就14 - MHD酸而言，松科与银杏种子脂质一样，是植物界中的主要例外情况，否则14 - MHD酸几乎仅存在于动物和微生物的脂质中。14 - MHD酸在木材和叶片脂质中也存在，但其生物合成及代谢作用仍不清楚。
