Tao Y X, Zhao Z Q
Shanghai Brain Research Institute, Chinese Academy of Sciences, People's Republic of China.
Neuropeptides. 1997 Aug;31(4):327-32. doi: 10.1016/s0143-4179(97)90067-6.
The presence of Fos-labeled neurons at ultrastructural level was confirmed in the spinal superficial laminae following an injection of formalin into rat hindpaw in the present study. The Fos-like immunoreactive products were found exclusively in regions associated with the euchromatin in the nuclei of Fos-labeled neurons. By the methods used-of anterograde transport of horseradish peroxidase conjugated to wheat-germ agglutinin and immunocytochemistry-it was observed that some Fos-labeled neuronal bodies received synaptic contacts from, or were apposed directly to, small diameter primary afferent terminals in the spinal superficial laminae. By means of double-labeled immunocytochemistry, a direct apposition was often observed and a synaptic relationship was occasionally found between Fos-labeled neuronal bodies and substance P-like immunoreactive terminals.
在本研究中，向大鼠后爪注射福尔马林后，在脊髓浅表层超微结构水平证实了Fos标记神经元的存在。Fos样免疫反应产物仅在Fos标记神经元细胞核中与常染色质相关的区域发现。通过使用与小麦胚芽凝集素偶联的辣根过氧化物酶顺行运输和免疫细胞化学方法，观察到一些Fos标记的神经元胞体接受来自脊髓浅表层小直径初级传入终末的突触接触，或与之直接并列。通过双重标记免疫细胞化学方法，经常观察到Fos标记的神经元胞体与P物质样免疫反应性终末之间直接并列，偶尔也发现突触关系。
