Barrett T L, Smith K J, Hodge J J, Butler R, Hall F W, Skelton H G
Department of Dermatology, Naval Medical Center, San Diego, California, USA.
J Am Acad Dermatol. 1997 Sep;37(3 Pt 1):430-7. doi: 10.1016/s0190-9622(97)70145-2.
Increased expression of p53 has been found in the majority of basal cell carcinomas (BCCs); however, UV-light-induced signature mutations are present in only about 50% of cases. Increased nuclear staining with an immunohistochemical marker of proliferation, proliferating cell nuclear antigen (PCNA), has been correlated with aggressive behavior in BCC.
Our purpose was to determine whether there is any relationship between different histologic variants of BCC and their expression of p53, PCNA, and Ki-67.
We used immunohistochemical stains for p53, PCNA, and Ki-67, in superficial-multicentric, nodular-noduloulcerative, sclerosing, infiltrative, and metatypical BCC, to determine whether the staining patterns differ in these different histologic variants of BCC.
Superficial-multicentric BCCs were negative for p53 in four of eight tumors. Nodular BCC showed moderately intense p53 nuclear staining with some peripheral accentuation. PCNA nuclear staining was greater than Ki-67, and PCNA-positive cells were fewer than 10% in nodular BCC. Sclerosing and infiltrative BCC showed intense p53 nuclear staining with peripheral accentuation. PCNA nuclear staining was greater than Ki-67, and PCNA-positive cells were greater than 30% in the majority of these tumors. Metatypical BCCs showed diffuse intense p53 staining. PCNA nuclear staining was greater than Ki-67, and PCNA-positive cells were greater than 30% in all tumors studied. When overlying actinic keratoses showed p53 staining, the staining did not necessarily correlate with the intensity or even the presence of positive staining in the subjacent BCC.
There are at least four distinctive patterns for staining of p53, PCNA, and Ki-67 that correlate with different histologic variants of BCC.
在大多数基底细胞癌（BCC）中发现p53表达增加；然而，紫外线诱导的特征性突变仅在约50%的病例中出现。增殖细胞核抗原（PCNA）作为一种增殖免疫组化标志物，其核染色增加与BCC的侵袭性行为相关。
我们的目的是确定BCC的不同组织学变体与其p53、PCNA和Ki-67表达之间是否存在任何关系。
我们对浅表多中心型、结节-结节溃疡型、硬化型、浸润型和异型BCC进行p53、PCNA和Ki-67免疫组化染色，以确定这些不同组织学变体的BCC染色模式是否不同。
8例肿瘤中有4例浅表多中心型BCC的p53呈阴性。结节型BCC显示p53核染色中等强度，外周有一些增强。PCNA核染色大于Ki-67，结节型BCC中PCNA阳性细胞少于10%。硬化型和浸润型BCC显示p53核染色强烈，外周增强。PCNA核染色大于Ki-67，在大多数这些肿瘤中PCNA阳性细胞大于30%。异型BCC显示弥漫性强烈的p53染色。PCNA核染色大于Ki-67，在所有研究的肿瘤中PCNA阳性细胞大于30%。当覆盖的光化性角化病显示p53染色时，该染色不一定与下方BCC的染色强度甚至阳性染色的存在相关。
p53、PCNA和Ki-67染色至少有四种不同模式，与BCC的不同组织学变体相关。
